This study investigates the enrichment of aptamers targeting the norovirus protruding domain in the presence of foods often associated with norovirus outbreaks. The goal is to explore if and how the presence of food alters in vitro selection of aptamers and target binding of the enriched oligonucleotides. Our study demonstrates that the introduction of food to SELEX (systematic evolution of ligands by exponential enrichment) is either detrimental to enrichment of oligonucleotides with target-specific binding, or facilitates enrichment of non-target-specific oligonucleotides. Moreover, a relationship between target binding of enriched oligonucleotides in presence of food and their selection condition was not observed. Our findings also suggest that a pathogen specific aptamer with application in food does not need to be selected in presence of the particular food, but may require properties beyond high affinity and selectivity to be applied for pathogen extraction and detection in undiluted food matrices.

这项研究调查了在存在通常与诺如病毒暴发相关的食物的情况下，针对诺如病毒突出结构域的适体的富集。目的是探索食物的存在是否以及如何在体外改变适体的选择和富集的寡核苷酸的靶结合。我们的研究表明，将食物引入SELEX（通过指数富集进行配体的系统进化）既不利于具有靶标特异性结合的寡核苷酸的富集，又有助于非靶标特异性寡核苷酸的富集。此外，在食物存在下，未观察到富集寡核苷酸的靶结合与它们的选择条件之间的关系。我们的发现还表明，不需要在存在特定食物的情况下选择在食物中应用的病原体特异性适体，但是可能需要超出高亲和力和选择性的特性才能应用于未稀释食物基质中的病原体提取和检测。