There is an unmet need for an optimal scaffold as cell transplantation carrier to induce corneal reconstruction. In this study, a blend membrane was prepared with carboxymethyl chitosan (CMCTS), gelatin, and hyaluronic acid. To investigate its cytocompatibility, primary rabbit corneal epithelial cells (CEpCs) were seeded on it and growth and proliferation were evaluated. The blend membrane was found to be transparent, biodegradable, and suitable for CEpCs attachment and proliferation, which could maintain the epithelial cell-like protein expression of CEpCs. The combination of CEpCs and CMCTS-blended membrane (CEpCs/CMCTS membrane) was used to treat alkali-induced corneal damage in rabbits and healing effects were evaluated by visual observation, slit lamp, hematoxylin-eosin and immunofluorescence staining. CEpCs/CMCTS membrane could improve corneal epithelial reconstruction significantly and restore cornea transparency and thickness. Hence, this combination treatment may serve as a rapid and effective way for corneal wound healing.

最佳支架作为细胞移植载体以诱导角膜重建的需求尚未得到满足。在这项研究中，用羧甲基壳聚糖（CMCTS），明胶和透明质酸制备了一种共混膜。为了研究其细胞相容性，将原代兔角膜上皮细胞（CEpCs）接种在其上并评估其生长和增殖。发现共混膜是透明的，可生物降解的并且适合CEpCs的附着和增殖，其可以维持CEpCs的上皮细胞样蛋白表达。CEpCs和CMCTS融合膜（CEpCs / CMCTS膜）的组合用于治疗碱引起的兔角膜损伤，并通过肉眼观察，裂隙灯，苏木精-曙红和免疫荧光染色评估其愈合效果。CEpCs / CMCTS膜可显着改善角膜上皮重建并恢复角膜透明性和厚度。因此，这种联合治疗可以作为角膜伤口愈合的快速有效方法。