Tris (2-butoxyethyl) phosphate (TBOEP) is an environmental contaminant that poses serious risks to aquatic organisms and their associated ecosystem. Recently, the reproductive and developmental toxicology of TBOEP has been reported. However, fewer studies have assessed the neurotoxic effects in zebrafish (Danio rerio) larvae. In this study, zebrafish embryos were subjected to waterborne exposure of TBOEP at 0, 50, 500, 1500 and 2500 μg/L from 2 to 144-h post-fertilization (hpf). Behavioral measurements showed that TBOEP exposure reduced embryonic spontaneous movement and decreased swimming speed of larvae in response to dark stimulation. In accordance with these motor effects, TBOEP treatment reduced neuron-specific GFP expression in transgenic Tg (HuC-GFP) zebrafish larvae and inhibited the growth of secondary motoneurons, as well as decreased expression of marker genes related to central nervous system development in TBOEP treated group. Furthermore, increased concentrations of reactive oxygen species (ROS) and malondialdehyde (MDA), as well as reduction of SOD activity were detected in TBOEP exposure group. The present results showed that the alteration in motor neuron and oxidative stress could together lead to the motor behavior alterations induced by TBOEP.

磷酸三（2-丁氧基乙基）酯（TBOEP）是一种环境污染物，会对水生生物及其相关的生态系统构成严重威胁。最近，已经报道了TBEOP的生殖和发育毒理学。但是，很少有研究评估斑马鱼（Danio rerio）幼虫的神经毒性作用。在这项研究中，斑马鱼胚胎在受精后2至144小时（hpf）分别以0、50、500、1500和2500μg/ L的水暴露于TBOEP。行为测量表明，暴露于TBOEP会降低幼虫对黑暗刺激的响应，从而降低其胚胎的自发运动并降低其游泳速度。根据这些马达效果，TBOEP治疗在转基因减少神经元特异性GFP表达的Tg（于克-GFP）斑马鱼幼虫，并抑制TBOEP治疗组中继发性运动神经元的生长，以及与中枢神经系统发育相关的标志物基因的表达降低。此外，在TBOEP暴露组中发现了活性氧（ROS）和丙二醛（MDA）浓度增加，以及SOD活性降低。目前的结果表明，运动神经元和氧化应激的改变可能共同导致由TBOEP诱导的运动行为改变。