Abstract This paper describes development and validation of rapid and sensitive pre-column derivatization HPLC method to determine free and total formaldehyde content in five hair straightening products purchased from the local market. None of these products was labeled to contain formaldehyde and all of them were labeled with specific formaldehyde releasers. The formaldehyde derivative was separated using a µBondapak™ C18 column with mobile phase consisted of acetonitrile: water (70:30, v/v) and detected at 350 nm. The method was linear over the concentration range of 8–80 ng mL−1 (r = 09997) with detection limit of 0.8 ng mL−1. The formaldehyde derivative was stable for 30 days at room temperature. The results showed that all the samples were formaldehyde positive. The concentrations of free and total formaldehyde were 0.015–3.336 g% and 1.462–3.877 g%, respectively. The method showed high precision as the values of relative standard deviations (RSD) did not exceed 2.20%.

中文翻译：

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柱前衍生化 HPLC 方法快速、灵敏地测定直发产品中的游离甲醛和总甲醛

摘要 本文描述了快速、灵敏的柱前衍生化 HPLC 方法的开发和验证，用于测定从当地市场购买的五种直发产品中的游离甲醛和总甲醛含量。这些产品都没有贴上含有甲醛的标签，所有这些产品都贴有特定的甲醛释放剂。使用由乙腈: 水 (70:30, v/v) 组成的流动相，使用 µBondapak™ C18 色谱柱分离甲醛衍生物，并在 350 nm 处检测。该方法在 8–80 ng mL-1 (r = 09997) 的浓度范围内呈线性，检测限为 0.8 ng mL-1。甲醛衍生物在室温下可稳定保存 30 天。结果表明，所有样品均呈甲醛阳性。游离甲醛和总甲醛的浓度分别为 0.015–3.336 g% 和 1.462–3。分别为 877 g%。该方法显示出高精度，因为相对标准偏差 (RSD) 值不超过 2.20%。