Integrative structure and functional anatomy of a nuclear pore complex,Nature

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Integrative structure and functional anatomy of a nuclear pore complex
Nature ( IF 50.5 ) Pub Date : 2018-03-01 , DOI: 10.1038/nature26003
Seung Joong Kim ₁ , Javier Fernandez-Martinez ₂ , Ilona Nudelman ₂ , Yi Shi ₃ , Wenzhu Zhang ₃ , Barak Raveh ₁ , Thurston Herricks ₄ , Brian D Slaughter ₅ , Joanna A Hogan ₆ , Paula Upla ₇ , Ilan E Chemmama ₁ , Riccardo Pellarin ₁ , Ignacia Echeverria ₁ , Manjunatha Shivaraju ₅ , Azraa S Chaudhury ₂ , Junjie Wang ₃ , Rosemary Williams ₂ , Jay R Unruh ₅ , Charles H Greenberg ₁ , Erica Y Jacobs ₃ , Zhiheng Yu ₈ , M Jason de la Cruz ₈ , Roxana Mironska ₂ , David L Stokes ₇ , John D Aitchison _{4,

9} , Martin F Jarrold ₆ , Jennifer L Gerton ₅ , Steven J Ludtke ₁₀ , Christopher W Akey ₁₁ , Brian T Chait ₃ , Andrej Sali ₁ , Michael P Rout ₂

Affiliation

Department of Bioengineering and Therapeutic Sciences, Department of Pharmaceutical Chemistry, and California Institute for Quantitative Biosciences, University of California, San Francisco, San Francisco, California 94158, USA.
Laboratory of Cellular and Structural Biology, The Rockefeller University, New York, New York 10065, USA.
Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, New York 10065, USA.
Institute for Systems Biology, 401 Terry Ave. N., Seattle, Washington 98109, USA.
Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA.
Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA.
Skirball Institute and Department of Cell Biology, New York University School of Medicine, New York, New York 10016, USA.
Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, Virginia 20148, USA.
Center for Infectious Disease Research, Seattle, Washington 98109, USA.
Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, 1 Baylor Plaza, Houston, Texas 77030, USA.
Department of Physiology and Biophysics, Boston University School of Medicine, 700 Albany Street, Boston, Massachusetts 02118, USA.

Nuclear pore complexes play central roles as gatekeepers of RNA and protein transport between the cytoplasm and nucleoplasm. However, their large size and dynamic nature have impeded a full structural and functional elucidation. Here we determined the structure of the entire 552-protein nuclear pore complex of the yeast Saccharomyces cerevisiae at sub-nanometre precision by satisfying a wide range of data relating to the molecular arrangement of its constituents. The nuclear pore complex incorporates sturdy diagonal columns and connector cables attached to these columns, imbuing the structure with strength and flexibility. These cables also tie together all other elements of the nuclear pore complex, including membrane-interacting regions, outer rings and RNA-processing platforms. Inwardly directed anchors create a high density of transport factor-docking Phe-Gly repeats in the central channel, organized into distinct functional units. This integrative structure enables us to rationalize the architecture, transport mechanism and evolutionary origins of the nuclear pore complex.

中文翻译：

核孔复合体的整体结构和功能解剖

核孔复合物在细胞质和核质之间作为 RNA 和蛋白质转运的看门人发挥着核心作用。然而，它们的大尺寸和动态特性阻碍了完整的结构和功能阐明。在这里，我们通过满足与其成分的分子排列相关的广泛数据，以亚纳米精度确定了酵母酿酒酵母的整个 552 蛋白质核孔复合物的结构。核孔复合体包含坚固的斜柱和连接到这些柱的连接电缆，使结构具有强度和灵活性。这些电缆还将核孔复合体的所有其他元素联系在一起，包括膜相互作用区域、外环和 RNA 处理平台。向内定向的锚在中央通道中产生高密度的运输因子对接 Phe-Gly 重复序列，组织成不同的功能单元。这种综合结构使我们能够使核孔复合体的结构、运输机制和进化起源合理化。

更新日期：2018-03-01

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