Protein–Polymer Microcapsules for PCR Technology,ChemBioChem

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Protein–Polymer Microcapsules for PCR Technology
ChemBioChem ( IF 2.6 ) Pub Date : 2018-04-27 , DOI: 10.1002/cbic.201800080
Xiaoliang Wang ₁ , Yang Liu ₁ , Jiao Liu ₂ , Zhijun Chen ₁

Affiliation

Recombinant mCherry protein was covalently linked with poly(N‐isopropylacrylamide) (PNIPAAm) to form an amphiphilic protein–polymer conjugate, which assembled into microcapsules at a water–oil interface. These microcapsule structures were thermoresistant and their use avoided the need for complex crosslinking and water–oil phase transitions. The heatproof microreactors can be used in polymerase chain reaction (PCR) technology.

中文翻译：

用于 PCR 技术的蛋白质聚合物微胶囊

重组 mCherry蛋白与聚（ N-异丙基丙烯酰胺）（PNIPAAm）共价连接，形成两亲性蛋白-聚合物缀合物，在水-油界面组装成微胶囊。这些微胶囊结构具有耐热性，并且它们的使用避免了复杂的交联和水油相变。耐热微反应器可用于聚合酶链式反应（PCR）技术。

更新日期：2018-04-27

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