Abstract Mycotoxin contamination of staple food commodities is a relevant health and economic issue worldwide. The development of green and effective reduction strategies to counteract the contamination by multiple mycotoxins has become an urgent need. The aim of this work was to evaluate the capability of a laccase (LC) from Pleurotus eryngii and a laccase-mediator systems (LMSs) to degrade aflatoxin B1 (AFB1), fumonisin B1 (FB1), ochratoxin A (OTA), deoxynivalenol (DON), Zearalenone (ZEN) and T-2 toxin in in vitro assays. In addition, the simultaneous mycotoxin degradation capability with selected LMSs was evaluated with combinations of AFB1 and ZEN, and FB1 and T-2 toxin. Redox mediators were found to drastically increasethe degradation efficiencies of the enzyme. AFB1, FB1, OTA, ZEN and T-2 toxin degradation by the best performing LMS were 73%, 74%, 27%, 100% and 40%, respectively. No degradation was registered for DON. Notably, AFB1 and ZEN were simultaneously degraded by 86% and 100%, while FB1 and T-2 by 25% and 100%, respectively. LMS proved to be a promising approach to enhance degradation properties of LC enzymes and for the potential development of a multi-mycotoxin reducing method.

中文翻译：

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杏鲍菇 Ery4 漆酶和氧化还原介质体外单一和联合降解霉菌毒素

摘要 主食商品的霉菌毒素污染是世界范围内一个相关的健康和经济问题。开发绿色有效的减少策略以抵消多种真菌毒素的污染已成为迫切需要。这项工作的目的是评估杏鲍菇漆酶 (LC) 和漆酶介体系统 (LMS) 降解黄曲霉毒素 B1 (AFB1)、伏马菌素 B1 (FB1)、赭曲霉毒素 A (OTA)、脱氧雪腐镰刀菌烯醇的能力。 DON)、玉米赤霉烯酮 (ZEN) 和 T-2 毒素在体外测定中。此外，还使用 ​​AFB1 和 ZEN 以及 FB1 和 T-2 毒素的组合评估了选定 LMS 的同时真菌毒素降解能力。发现氧化还原介质可显着提高酶的降解效率。性能最好的 LMS 对 AFB1、FB1、OTA、ZEN 和 T-2 毒素的降解率为 73%，分别为 74%、27%、100% 和 40%。没有记录 DON 的降解。值得注意的是，AFB1 和 ZEN 同时降解了 86% 和 100%，而 FB1 和 T-2 分别降解了 25% 和 100%。LMS 被证明是一种很有前途的方法，可以增强 LC 酶的降解特性，并有可能开发出一种减少多真菌毒素的方法。