Fast Determination of Antioxidant Capacity of Food Samples Using Continuous Amperometric Detection on Polyester Screen‐printed Graphitic Electrodes,Electroanalysis

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Fast Determination of Antioxidant Capacity of Food Samples Using Continuous Amperometric Detection on Polyester Screen‐printed Graphitic Electrodes
Electroanalysis ( IF 2.7 ) Pub Date : 2018-03-14 , DOI: 10.1002/elan.201800122
Iana V. S. Arantes ₁ , Jéssica S. Stefano ₁ , Raquel M. F. Sousa ₁ , Eduardo M. Richter ₁ , Christopher W. Foster ₂ , Craig E. Banks ₂ , Rodrigo A. A. Muñoz ₁

Affiliation

The determination of antioxidant capacity of food samples using disposable polyester screen‐printed graphitic macroelectrodes (SPGE) coupled with a batch‐injection cell based on the measurement of the consumption of 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH^.) is reported. The SPGEs are organic‐resistant electrodes and thus compatible with food samples and organic solvents used to dissolve DPPH^.. A micropipette controlled the release (193 μL s⁻¹) of sample (150 μL) upon the sensor/working electrode immersed in electrolyte (1 : 1 ethanol/methanol containing 0.05 mol L⁻¹ LiCl). The first demonstration of this protocol was devoted to the analysis of edible oils. The remaining DPPH^. was amperometrically detected at +0.1 V (vs. pseudo AgCl). The antioxidant capacity (percentage of DPPH^. scavenging and mg equivalent of tocopherol) was obtained using the proposed and spectrophotometric methods. The results were in agreement according to t‐test (confidence level of 95 %). The method is precise (RSD=2.3 %, n=12), fast (180 h⁻¹), presents low detection limit of DPPH^. (1 μmol L⁻¹), and allows on‐site analysis. Moreover, the proposed method can be extended to the analysis of antioxidant capacity of other samples of agricultural and food interests.

中文翻译：

聚酯丝网印刷石墨电极上连续安培检测法快速测定食品的抗氧化能力

确定的使用加上基于2,2-二苯基-1-苦基肼的消耗的测量的分批注入细胞一次性聚酯丝网印刷石墨macroelectrodes（SPGE）食物样品的抗氧化能力（DPPH ^。报道）。SPGE是耐有机电极，因此可与食品样本和用于溶解DPPH的有机溶剂兼容^。。甲微量受控的释放（193μL小号^-1时浸渍在电解液中的传感器/工作电极的样品）（150μL）（1：含有0.05摩尔大号1乙醇/甲醇^-1的LiCl）。该协议的首次演示专门用于食用油的分析。剩下的DPPH ^。在+0.1 V（相对于伪AgCl）进行电流分析检测。的抗氧化能力（DPPH的百分比^。使用所提出的法和分光光度法得到清扫和生育酚的毫克当量）。根据t检验，结果一致（置信度为95％）。该方法精确（RSD = 2.3％，n = 12），快速（180h ^-1），显示了DPPH的低检测限^。（1μmolL ^-1），并允许进行现场分析。此外，该方法可以扩展到其他农业和食品利益样品的抗氧化能力分析。

更新日期：2018-03-14

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