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An Ultrasensitive Fluorescence Sensor with Simple Operation for Cu2+ Specific Detection in Drinking Water
ACS Omega ( IF 3.7 ) Pub Date : 2018-03-14 00:00:00 , DOI: 10.1021/acsomega.7b01497 Jinting Gao 1 , Jinjin Yin 1 , Zhanhui Tao 1 , Yaqing Liu 1 , Xiaodong Lin 1 , Jiankang Deng 1 , Shuo Wang 1, 2
ACS Omega ( IF 3.7 ) Pub Date : 2018-03-14 00:00:00 , DOI: 10.1021/acsomega.7b01497 Jinting Gao 1 , Jinjin Yin 1 , Zhanhui Tao 1 , Yaqing Liu 1 , Xiaodong Lin 1 , Jiankang Deng 1 , Shuo Wang 1, 2
Affiliation
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Whether short-term or long-term, overexposure to an abnormal amount of copper ion does significant harm to human health. Considering its nonbiodegradability, it is critical to sensitively detect copper ion. Herein, a novel fluorescent strategy with a “turn-on” signal was developed for highly sensitive and specific detection of copper ion (Cu2+). In the present investigation, we found that Cu2+ exhibits excellent peroxidase-like catalytic activity toward oxidizing the nonfluorescent substrate of Amplex Red into the product of resofurin with outstanding fluorescence emission under the aid of H2O2. Thus, an enzyme-free and label-free sensing system was constructed for copper ion detection with quite simple operation. To ensure the detection sensitivity and reproducibility, the amount of H2O2 and incubation time were optimized. The limit of detection can reach as low as 1.0 nM. In addition, the developed assay demonstrated excellent specificity and could be utilized to detect copper ion in water samples including tap water and bottled purified water without standing recovery.
中文翻译：
操作简单的超灵敏荧光传感器，用于饮用水中 Cu2+ 的特异性检测
无论是短期还是长期，过量接触异常量的铜离子都会对人体健康造成重大危害。考虑到其不可生物降解性，灵敏地检测铜离子至关重要。在此，开发了一种具有“开启”信号的新型荧光策略，用于高灵敏度和特异性地检测铜离子（Cu 2+）。在本研究中，我们发现Cu 2+表现出优异的类过氧化物酶催化活性，在H 2 O 2的帮助下将Amplex Red的非荧光底物氧化成具有出色荧光发射的试呋林产物。因此，构建了一种操作非常简单的无酶、无标记的铜离子检测传感系统。为保证检测灵敏度和重现性，对H 2 O 2用量和孵育时间进行了优化。检测限可低至1.0 nM。此外，所开发的检测方法表现出优异的特异性，可用于检测水样中的铜离子，包括自来水和瓶装纯净水，无需静置回收。
更新日期：2018-03-14
中文翻译：
操作简单的超灵敏荧光传感器，用于饮用水中 Cu2+ 的特异性检测
无论是短期还是长期，过量接触异常量的铜离子都会对人体健康造成重大危害。考虑到其不可生物降解性，灵敏地检测铜离子至关重要。在此，开发了一种具有“开启”信号的新型荧光策略，用于高灵敏度和特异性地检测铜离子（Cu 2+）。在本研究中，我们发现Cu 2+表现出优异的类过氧化物酶催化活性，在H 2 O 2的帮助下将Amplex Red的非荧光底物氧化成具有出色荧光发射的试呋林产物。因此，构建了一种操作非常简单的无酶、无标记的铜离子检测传感系统。为保证检测灵敏度和重现性，对H 2 O 2用量和孵育时间进行了优化。检测限可低至1.0 nM。此外，所开发的检测方法表现出优异的特异性，可用于检测水样中的铜离子，包括自来水和瓶装纯净水，无需静置回收。
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