AbstractHollow carbon dots (HCDs) were prepared by a solvothermal method and conjugated to either tetramethyl rhodamine isothiocyanate (TRITC) or fluorescein-5-isothiocyanate (FITC). This resulted in HCDs with bright red or green fluorescence, with excitation/emission peaks at 550/580 and 491/520 nm, respectively. The nanocomposites are well water-soluble, remarkably photostable and biocompatible. In addition, the fluorescence of the composites is more stable in a reactive oxygen environment than the free dyes. Confocal images indicate that the nanoparticles quickly enter A549 cells and mainly accumulate in the cytoplasm. The wavelength of functionalized HCDs can be regulating via coupling the HCDs to different dyes. These results demonstrate that these composite materials can be very promising reagents for biological labeling and imaging. Graphical abstractSchematic of the preparation of hollow carbon dots conjugated to tetramethyl rhodamine isothiocyanate (RHCDs) by solvothermal method. The material is water-soluble, remarkably photostable and biocompatible. It was applied to cellular labeling and imaging.

中文翻译：

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用 FITC 或 TRITC 标记的空心碳点用于荧光细胞成像

摘要 通过溶剂热法制备了中空碳点 (HCD)，并与四甲基罗丹明异硫氰酸酯 (TRITC) 或荧光素 5-异硫氰酸酯 (FITC) 共轭。这导致 HCD 具有明亮的红色或绿色荧光，激发/发射峰分别位于 550/580 和 491/520 nm。纳米复合材料具有良好的水溶性、显着的光稳定性和生物相容性。此外，复合材料的荧光在活性氧环境中比游离染料更稳定。共聚焦图像表明纳米颗粒迅速进入 A549 细胞并主要积聚在细胞质中。功能化 HCD 的波长可以通过将 HCD 与不同染料偶联来调节。这些结果表明，这些复合材料可以成为非常有前途的生物标记和成像试剂。图形摘要通过溶剂热法制备与四甲基罗丹明异硫氰酸酯 (RHCD) 共轭的空心碳点的示意图。该材料是水溶性的，具有显着的光稳定性和生物相容性。它被应用于细胞标记和成像。