An analytical procedure for the analysis of 10 β-adrenergic agonists (cimaterol, terbutaline, salbutamol, isoxsuprine, ractopamine, cimbuterol, clenbuterol, brombuterol, mabuterol and mapenterol) in pork meat was developed and validated using LC-MS/MS. An automated dispersive pipette extraction (DPX) was employed on a Hamilton Microlab® NIMBUS96® platform to extract the analytes of interest prior to LC-MS/MS analysis. The extraction time was <20 min with a total LC-MS/MS run time of 9.6 min. The method was fully validated in accordance with the international guidelines (European Commission Decision 2002/657/EC and National Standards of People's Republic of China, GB/T 22286-2008) for limit of detection, limit of quantitation, carryover, extraction efficiency, matrix effects, linearity, and within and between-run precision. The proposed method can be successfully used in the routine determination of 10 β-adrenergic agonists in pork and as a potential solution for compliance monitoring in regulatory laboratories.

开发了一种用于分析猪肉中10种β-肾上腺素能激动剂（西马特罗，特布他林，沙丁胺醇，异硫磺嘌呤，莱克多巴胺，辛比妥尔，盐酸克仑特罗，溴化叔丁醇，马布特罗和Mapenterol）的分析程序，并使用LC-MS / MS进行了验证。在LC-MS / MS分析之前，在HamiltonMicrolab®NIMBUS96®平台上使用自动分散移液管萃取（DPX）来萃取目标分析物。萃取时间<20分钟，总LC-MS / MS运行时间为9.6分钟。该方法已根据国际准则（欧盟委员会第2002/657 / EC号决定和中华人民共和国国家标准，GB / T 22286-2008）进行了充分验证，包括检测限，定量限，残留，萃取效率，矩阵效应，线性度以及行程内和行程间精度。