The construction and biofunctionalization of the poly (Ɛ-caprolactone) (PCL)-chitosan (CHIT) nanofibrous mats, which included Polyamidoamine (PAMAM) dendrimer modified montmorillonite (Mt), for the cell adhesion and electrochemical cytosensing were accomplished in this report. After the intercalation of the PAMAM generation zero dendrimer into the Mt, PAMAM-Mt decorated PCL-CHIT electrospun nanofibers were formed. The addition of PAMAM caused the decrease of contact angle of PCL-CHIT nanofibers. The covalent immobilization of a tripeptide namely Arginylglycylaspartate (RGD) on both the PCL-CHIT/Mt and PCL-CHIT/PAMAM-Mt surface was carried out. U87-MG and HaCaT (negative control) cell lines were incubated on the PCL-CHIT/Mt/RGD and PCL-CHIT/PAMAM-Mt/RGD. The proliferation studies and imaging of the cells were carried out on these fibers. Finally, electrochemical measurements were performed after each modification step by differential pulse/cyclic voltammetry and electrochemical impedance spectroscopy. U87-MG cells were grown better than HaCaT cells on the PCL-CHIT/PAMAM-Mt/RGD surfaces. To the best of our knowledge, there is no study that developed electrochemical cytosensor using electrospun nanofibers as a cell adhesion platform.

本报告完成了聚（ε-己内酯）（PCL）-壳聚糖（CHIT）纳米纤维垫的构建和生物功能化，其中包括聚酰胺酰胺（PAMAM）树枝状聚合物修饰的蒙脱石（Mt），以实现细胞粘附和电化学细胞传感。在将PAMAM一代零树枝状聚合物插入到Mt中之后，形成了PAMAM-Mt装饰的PCL-CHIT电纺纳米纤维。PAMAM的加入导致PCL-CHIT纳米纤维的接触角减小。在PCL-CHIT / Mt和PCL-CHIT / PAMAM-Mt表面上共价固定了三肽即精氨酰糖基天冬氨酸（RGD）。将U87-MG和HaCaT（阴性对照）细胞系在PCL-CHIT / Mt / RGD和PCL-CHIT / PAMAM-Mt / RGD上孵育。在这些纤维上进行了细胞的增殖研究和成像。最后，在每个修饰步骤之后，通过差动脉冲/循环伏安法和电化学阻抗谱法进行电化学测量。在PCL-CHIT / PAMAM-Mt / RGD表面上，U87-MG细胞的生长优于HaCaT细胞。据我们所知，尚无研究开发使用电纺纳米纤维作为细胞粘附平台的电化学细胞传感器。