当前位置：
X-MOL 学术
›
Anal. Chem.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Exploring the Complexity of Steviol Glycosides Analysis Using Ion Mobility Mass Spectrometry
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-03-14 00:00:00 , DOI: 10.1021/acs.analchem.7b05002 Michael McCullagh 1 , David Douce 1 , Els Van Hoeck 2 , Severine Goscinny 2
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-03-14 00:00:00 , DOI: 10.1021/acs.analchem.7b05002 Michael McCullagh 1 , David Douce 1 , Els Van Hoeck 2 , Severine Goscinny 2
Affiliation
|
A proof of principle method using ion mobility-mass spectrometry (IM-MS) and collision induced dissociation (CID) coupled with micro flow ultra high-performance chromatography (UHPLC-IM-MS) has been developed to screen for steviol glycosides. Traveling wave ion mobility was used to determine rotationally averaged collision cross sections in nitrogen buffer gas (TWCCSN2). To explore the evolving applicability of ion mobility screening, the analytical approach was initially developed and applied to the analysis of a steviol/steviol glycoside spiked chocolate spread extract. Subsequently 55 food commodities were screened using a steviol glycoside TWCCSN2 library. IM analyses produced TWCCSN2 values, enabling the unequivocal identification of the steviol glycosides and isomeric pairs (negating the reliance on product ions). In addition, coeluting isomeric species, comprising (labile fragment ions, doubly charged dimers, and multiply charged species) have been identified and resolved. Isomeric false detections were avoided, with the coeluting isomeric species quantified. A quantitative assessment of TWCCSN2 in the analysis of steviol glycosides was performed.
中文翻译：
探索使用离子淌度质谱分析甜菊醇糖苷的复杂性
已经开发出使用离子淌度质谱（IM-MS）和碰撞诱导解离（CID）结合微流超高效色谱（UHPLC-IM-MS）的原理验证方法来筛选甜菊糖苷。使用行波离子迁移率来确定氮气缓冲气体（TW CCSN 2）中的旋转平均碰撞截面。为了探索离子迁移筛查的不断发展的适用性，最初开发了分析方法，并将其用于甜菊醇/甜菊醇糖苷加标巧克力酱提取物的分析。随后，使用甜菊糖苷TW CCSN 2文库筛选了55种食品。IM分析产生了TW CCSN 2值，可以明确鉴定甜菊糖苷和同分异构体对（消除了对产物离子的依赖）。此外，共洗脱的同分异构物质，包括（不稳定的碎片离子，双电荷的二聚体和多电荷的物质）已得到鉴定和解决。避免了同等的错误检测，并定量了共洗脱的异构体。在甜菊糖苷的分析中对TW CCSN 2进行了定量评估。
更新日期：2018-03-14
中文翻译：
探索使用离子淌度质谱分析甜菊醇糖苷的复杂性
已经开发出使用离子淌度质谱（IM-MS）和碰撞诱导解离（CID）结合微流超高效色谱（UHPLC-IM-MS）的原理验证方法来筛选甜菊糖苷。使用行波离子迁移率来确定氮气缓冲气体（TW CCSN 2）中的旋转平均碰撞截面。为了探索离子迁移筛查的不断发展的适用性，最初开发了分析方法，并将其用于甜菊醇/甜菊醇糖苷加标巧克力酱提取物的分析。随后，使用甜菊糖苷TW CCSN 2文库筛选了55种食品。IM分析产生了TW CCSN 2值，可以明确鉴定甜菊糖苷和同分异构体对（消除了对产物离子的依赖）。此外，共洗脱的同分异构物质，包括（不稳定的碎片离子，双电荷的二聚体和多电荷的物质）已得到鉴定和解决。避免了同等的错误检测，并定量了共洗脱的异构体。在甜菊糖苷的分析中对TW CCSN 2进行了定量评估。
京公网安备 11010802027423号