Negative Selection by Spiral Inertial Microfluidics Improves Viral Recovery and Sequencing from Blood,Analytical Chemistry

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Negative Selection by Spiral Inertial Microfluidics Improves Viral Recovery and Sequencing from Blood
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-03-14 00:00:00 , DOI: 10.1021/acs.analchem.7b05200
Kyungyong Choi , Hyunryul Ryu , Katherine J. Siddle _{1,

2} , Anne Piantadosi _{1,

3} , Lisa Freimark ₁ , Daniel J. Park ₁ , Pardis Sabeti _{1,

2,

4,

5} , Jongyoon Han ₆

Affiliation

In blood samples from patients with viral infection, it is often important to separate viral particles from human cells, for example, to minimize background in performing viral whole genome sequencing. Here, we present a microfluidic device that uses spiral inertial microfluidics with continuous circulation to separate host cells from viral particles and free nucleic acid. We demonstrate that this device effectively reduces white blood cells, red blood cells, and platelets from both whole blood and plasma samples with excellent recovery of viral nucleic acid. Furthermore, microfluidic separation leads to greater viral genome coverage and depth, highlighting an important application of this device in processing clinical samples for viral genome sequencing.

中文翻译：

螺旋惯性微流控技术的阴性选择可提高血液的病毒回收率和测序效率

在病毒感染患者的血液样本中，通常重要的是从人类细胞中分离病毒颗粒，例如，以最小化进行病毒全基因组测序的背景。在这里，我们介绍了一种微流体装置，该装置使用具有连续循环的惯性螺旋惯性微流体来将宿主细胞与病毒颗粒和游离核酸分开。我们证明了该设备可有效减少全血和血浆样品中的白细胞，红细胞和血小板，并具有出色的病毒核酸回收率。此外，微流体分离导致更大的病毒基因组覆盖范围和深度，突显了该设备在处理用于病毒基因组测序的临床样品中的重要应用。

更新日期：2018-03-14

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