The present study described a novel fast, simple and green one-pot synthesis approach of flower-shaped gold nanoparticles (flower-shaped AuNPs) by using antioxidant plant extracts. The AuNPs synthesized by Alchemilla mollis extract showed maximum absorbance at 610 nm and were polydispersed flower-shaped with average size (±SD) of 68.7 ± 2.8 nm. We have explored a colorimetric sensing assay for rapid detecting Listeria monocytogenes by using single-stranded DNA (ssDNA) conjugated flower-shaped AuNPs. ssDNA were used as probe for DNA detection and the synthesized flower-shaped AuNPs were used as indicators. The limit of detection for hlyA gene and genomic DNA of Listeria monocytogenes were 48.4 ng and 100.4 ng, respectively. The proposed assay is fast and specific for Listeria monocytogenes detection, with the improved color change and stability.

本研究描述了一种新颖，快速，简单且绿色的一锅法合成方法，该方法通过使用抗氧化剂植物提取物来合成花形金纳米颗粒（花形AuNPs）。炼金术提取物合成的AuNPs在610 nm处显示最大吸光度，呈多分散花状，平均大小（±SD）为68.7±2.8 nm。我们已经探索了比色传感测定法，通过使用单链DNA（ssDNA）缀合的花形AuNPs快速检测单核细胞增生李斯特菌。将ssDNA用作DNA检测的探针，并将合成的花形AuNPs用作指示剂。单核细胞增生性李斯特菌hlyA基因和基因组DNA的检出限分别为48.4 ng和100.4 ng。所提出的测定快速且特异性地用于单核细胞增生李斯特氏菌的检测，具有改善的颜色变化和稳定性。