The combination of nanoparticles and biomarkers yields functional nanostructured biointerface, which is playing a notable role in biotechnology development. Due to the 5-fold twined structure in the Au-Pt star-shaped decahedra not only allowed it to act as efficient scaffold for immobilization of antibody, but it also exhibits superior electrocatalytic activity toward H₂O₂ reduction, the nanocrystal as the efficient signal transduction label is first employed to construct an electrochemical immunosensor. Donor–π–Acceptor (D–π–A) linking fashion generates a dipolar push-pull system and assures superior intramolecular charge transfer. It is considered as a suitable π-conjugated backbone for conducting polymer on biointerface application. Under a D–π–A architecture which imidazole as the π-bridge and amino phenyl/phenyl groups as peripheral electron-donating/withdrawing functional groups, 4-(2,4,5-triphenyl-1H-imidazol-1-yl) aniline (TPIDA) is designed and synthesized for good biocompatibility and high conductivity. In this proposal, we attempt to integrate the above-mentioned two features from nanobiotechnology and organic bioelectronics. Then, a novel nonenzymatic sandwich-type immunosensor is performed by Au-Pt core-shell with surface-engineered twinning as a label and π-conjugated D–π–A polymers as the signal amplification platform. Human IgG (HIgG) as the model target protein can be detected with a wide linear range from 0.1 pg mL⁻¹ to 100 ng mL⁻¹. The detection limit is down to 0.06 pg mL⁻¹ (S/N = 3). Moreover, as a practical application, the prepared biosensor is used to monitor HIgG level in human serum with desirable results obtained.

纳米颗粒和生物标志物的结合产生功能性的纳米结构生物界面，这在生物技术发展中起着重要作用。由于Au-Pt星形十面体中的5倍孪生结构，不仅使其可用作固定抗体的有效支架，而且还表现出对H ₂ O _2的优异电催化活性。还原时，首先使用纳米晶体作为有效的信号转导标记来构建电化学免疫传感器。施主-π-受体（D-π-A）的连接方式产生了一个偶极推挽系统，并确保了优异的分子内电荷转移。它被认为是用于在生物界面应用中传导聚合物的合适的π共轭骨架。在D–π–A结构下，咪唑为π桥，氨基苯基/苯基为供电子/吸电子的外围官能团，4-（2,4,5-三苯基-1H-咪唑-1-基）苯胺（TPIDA）的设计和合成具有良好的生物相容性和高电导率。在此提案中，我们尝试将纳米生物技术和有机生物电子学的上述两个功能整合在一起。然后，Au-Pt核壳以表面工程孪晶为标记，π共轭D–π–A聚合物为信号放大平台，完成了一种新型的非酶三明治型免疫传感器。可以在0.1 pg / mL的宽线性范围内检测到人类IgG（HIgG）作为模型目标蛋白^-1至100 ng mL ^-1。检出限降至0.06 pg mL ^-1（S / N = 3）。此外，作为实际应用，所制备的生物传感器用于监测人血清中的HIgG水平，并获得理想的结果。