Water molecules (H₂O) often reduce luminescence lifetimes of various luminescence probes. The change of lifetime is usually caused by dynamic luminescence quenching induced by O-H oscillators which effectively take away energy from excited molecule. The process can be described by Stern-Volmer equation. We have studied selected luminescence systems where it is possible to detect considerable changes of lifetime in presence/absence of H₂O and D₂O in this work for analytical purposes. We have tested both, inorganic (Ln³⁺) and organic compounds using three different instrumentation in order to find the largest change between τ_H and τ_D. The Ln³⁺ containing systems have shown considerable increase/decrease of lifetimes in the presence/absence of D₂O (Eu³⁺: τ_D/τ_H = 34.5) whereas organic systems gave significantly lower values of τ_D/τ_H (coumarin 123 lifetime ratio, τ_D/τ_H = 1.94). The calculated LOD varied from 0.04 mol l⁻¹ (samarium nitrate) to 6.55 mol l⁻¹ (riboflavin).

水分子（H ₂ O）通常会缩短各种发光探针的发光寿命。寿命的变化通常是由OH振荡器引起的动态发光猝灭引起的，该猝灭有效地从激发的分子中夺走了能量。该过程可以用斯特恩-沃尔默方程式描述。我们已经研究了选定的发光系统，在该系统中，出于分析目的，可以检测存在/不存在H ₂ O和D ₂ O时寿命的显着变化。我们已经测试了两个，无机（LN ³⁺），并使用三种不同的仪器，以便找到之间的最大变化有机化合物τ _ħ和τ _d。Ln ³⁺含系统已经显示相当大的增加/在存在/不存在的d寿命的减少₂ O（铕³⁺：τ _d /τ _ħ = 34.5），而有机体系，得到的显著较低值τ _d /τ _ħ（香豆素123寿命比，τ _d /τ _ħ = 1.94）。计算的LOD从0.04mol l ^-1（硝酸sa）变化到6.55 mol l ^-1（核黄素）。