A novel and simple biosensor for the determination of bisphenol A (BPA) based on xanthine oxidase (XOD) enzymatic inhibition has been developed. The biosensor was prepared from xanthine oxidase immobilised by crosslinking with glutaraldehyde, with hypoxanthine as enzyme substrate, and was successfully applied to the determination of BPA using fixed potential amperometry. Biosensor performance was optimised with respect to the applied potential, influence of pH of the electrolyte solution, XOD loading and the substrate concentration. The enzyme inhibition mechanism was evaluated from Cornish-Bowden plus Dixon plots and was found to be reversible and competitive with an apparent inhibition constant of 8.15 nM. Under optimised conditions, the determination of BPA can be achieved in the linear range up to 41 nM with a detection limit of 1.0 nM, which is equal to the lowest reported in the literature, with very good repeatability and reproducibility. The selectivity of the biosensor was evaluated by performing an interference study and found to be excellent; and stability was investigated. It was successfully applied to the detection of BPA in mineral water and in river water.

基于黄嘌呤氧化酶（XOD）酶抑制作用的新型，简单的双酚A（BPA）生物传感器的开发。该生物传感器是由以黄嘌呤为酶底物，通过与戊二醛交联而固定化的黄嘌呤氧化酶制备的，并成功地用于固定电位安培法测定双酚A。针对所施加的电势，电解质溶液的pH值，XOD上样量和底物浓度，优化了生物传感器的性能。从康沃尔-鲍登（Cornish-Bowden）和迪克森（Dixon）地块评估了酶抑制机制，发现该酶抑制机制是可逆的且具有竞争性，表观抑制常数为8.15 nM。在最佳条件下，可以在高达41 nM的线性范围内实现BPA的测定，检测限为1.0 nM，它等于文献中报道的最低值，具有很好的可重复性和可重复性。通过进行干扰研究评估了生物传感器的选择性，发现其极佳；和稳定性进行了调查。它已成功应用于矿泉水和河水中的双酚A的检测。