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Comparative estrogenicity of endogenous, environmental and dietary estrogens in pregnant women I: Serum levels, variability and the basis for urinary biomonitoring of serum estrogenicity
Food and Chemical Toxicology ( IF 3.9 ) Pub Date : 2018-03-13 , DOI: 10.1016/j.fct.2018.03.017
Stefanie C. Fleck , Nathan C. Twaddle , Mona I. Churchwell , Daniel R. Doerge , Paritosh Pande , Justin G. Teeguarden

Biomonitoring of human exposure to estrogens most frequently focuses on environmental and dietary estrogens, and infrequently includes measures of exposure to potent endogenous estrogens present in serum. Pregnancy is a developmentally sensitive period during which “added” serum estrogenicity exceeding normal intra-individual daily variability may be of particular relevance. We made repeated measurements of serum concentrations of estrone (E1), estradiol (E2), estriol (E3), estetrol (E4), daidzein (DDZ), genistein (GEN) and bisphenol A (BPA) in thirty pregnant women using ultra-performance liquid chromatography coupled with tandem mass spectrometry detection (UPLC-MS/MS) and electrospray ionization (ESI). Serum E1, E2, and E3 concentrations varied significantly (coefficients of variation 9–10%) with broad ranges across the cohort: 1.61–85.1 nM, 9.09–69.7 nM, and 1.5–36.3 nM respectively. BPA (undetected, estimated from total exposure), DDZ and GEN concentrations were 1-5 orders of magnitude lower. The 24-h urinary elimination profiles of endogenous estrogens were each strongly correlated with their corresponding serum concentrations (Pearson's Correlation Coefficients of 0.83 (E1), 0.84 (E2) and 0.94 (E3)). A multivariate regression analysis produced equations for estimating serum concentrations of E1, E2, E3, E4, GEN and DDZ from urinary elimination rates and gestation period, an important step towards non-invasive biomonitoring for assessment of “added” estrogenicity during pregnancy.



中文翻译:

孕妇内源性,环境性和饮食性雌激素的比较雌激素性I:血清水平,变异性和尿液生物监测血清雌激素性的基础

人类对雌激素暴露的生物监测最常侧重于环境和饮食中的雌激素,并且很少包括对血清中存在的强力内源性雌激素暴露的测量。怀孕是一个发育敏感的时期,在此期间,“增加的”血清雌激素水平超过正常的个体内日常变化可能是特别重要的。我们使用超高效液相色谱法重复测量了30名孕妇的血清雌酮(E1),雌二醇(E2),雌三醇(E3),雌二醇(E4),大豆黄酮(DDZ),染料木黄酮(GEN)和双酚A(BPA)的浓度。高效液相色谱,加上串联质谱检测(UPLC-MS / MS)和电喷雾电离(ESI)。血清E1,E2和E3的浓度差异很大(变异系数9-10%),整个队列范围广泛:1.61-85.1 nM,9。分别为09-69.7 nM和1.5-36.3 nM。BPA(未检出,根据总暴露量估算),DDZ和GEN浓度降低1-5个数量级。内源性雌激素的24小时尿液排泄曲线均与它们相应的血清浓度密切相关(皮尔森相关系数为0.83(E1),0.84(E2)和0.94(E3))。多元回归分析产生了方程,用于根据排尿率和妊娠期来估算E1,E2,E3,E4,GEN和DDZ的血清浓度,这是朝着非侵入性生物监测评估怀孕期间“增加的”雌激素性迈出的重要一步。内源性雌激素的24小时尿液排泄曲线均与它们相应的血清浓度密切相关(皮尔森相关系数为0.83(E1),0.84(E2)和0.94(E3))。多元回归分析产生了方程,用于根据排尿率和妊娠期来估算E1,E2,E3,E4,GEN和DDZ的血清浓度,这是朝着非侵入性生物监测评估怀孕期间“增加的”雌激素性迈出的重要一步。内源性雌激素的24小时尿液排泄曲线均与它们相应的血清浓度密切相关(皮尔森相关系数为0.83(E1),0.84(E2)和0.94(E3))。多元回归分析产生了方程,用于根据排尿率和妊娠期来估算E1,E2,E3,E4,GEN和DDZ的血清浓度,这是朝着非侵入性生物监测评估怀孕期间“增加的”雌激素性迈出的重要一步。

更新日期:2018-03-13
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