Biomonitoring of human exposure to estrogens most frequently focuses on environmental and dietary estrogens, and infrequently includes measures of exposure to potent endogenous estrogens present in serum. Pregnancy is a developmentally sensitive period during which “added” serum estrogenicity exceeding normal intra-individual daily variability may be of particular relevance. We made repeated measurements of serum concentrations of estrone (E1), estradiol (E2), estriol (E3), estetrol (E4), daidzein (DDZ), genistein (GEN) and bisphenol A (BPA) in thirty pregnant women using ultra-performance liquid chromatography coupled with tandem mass spectrometry detection (UPLC-MS/MS) and electrospray ionization (ESI). Serum E1, E2, and E3 concentrations varied significantly (coefficients of variation 9–10%) with broad ranges across the cohort: 1.61–85.1 nM, 9.09–69.7 nM, and 1.5–36.3 nM respectively. BPA (undetected, estimated from total exposure), DDZ and GEN concentrations were 1-5 orders of magnitude lower. The 24-h urinary elimination profiles of endogenous estrogens were each strongly correlated with their corresponding serum concentrations (Pearson's Correlation Coefficients of 0.83 (E1), 0.84 (E2) and 0.94 (E3)). A multivariate regression analysis produced equations for estimating serum concentrations of E1, E2, E3, E4, GEN and DDZ from urinary elimination rates and gestation period, an important step towards non-invasive biomonitoring for assessment of “added” estrogenicity during pregnancy.

人类对雌激素暴露的生物监测最常侧重于环境和饮食中的雌激素，并且很少包括对血清中存在的强力内源性雌激素暴露的测量。怀孕是一个发育敏感的时期，在此期间，“增加的”血清雌激素水平超过正常的个体内日常变化可能是特别重要的。我们使用超高效液相色谱法重复测量了30名孕妇的血清雌酮（E1），雌二醇（E2），雌三醇（E3），雌二醇（E4），大豆黄酮（DDZ），染料木黄酮（GEN）和双酚A（BPA）的浓度。高效液相色谱，加上串联质谱检测（UPLC-MS / MS）和电喷雾电离（ESI）。血清E1，E2和E3的浓度差异很大（变异系数9-10％），整个队列范围广泛：1.61-85.1 nM，9。分别为09-69.7 nM和1.5-36.3 nM。BPA（未检出，根据总暴露量估算），DDZ和GEN浓度降低1-5个数量级。内源性雌激素的24小时尿液排泄曲线均与它们相应的血清浓度密切相关（皮尔森相关系数为0.83（E1），0.84（E2）和0.94（E3））。多元回归分析产生了方程，用于根据排尿率和妊娠期来估算E1，E2，E3，E4，GEN和DDZ的血清浓度，这是朝着非侵入性生物监测评估怀孕期间“增加的”雌激素性迈出的重要一步。内源性雌激素的24小时尿液排泄曲线均与它们相应的血清浓度密切相关（皮尔森相关系数为0.83（E1），0.84（E2）和0.94（E3））。多元回归分析产生了方程，用于根据排尿率和妊娠期来估算E1，E2，E3，E4，GEN和DDZ的血清浓度，这是朝着非侵入性生物监测评估怀孕期间“增加的”雌激素性迈出的重要一步。内源性雌激素的24小时尿液排泄曲线均与它们相应的血清浓度密切相关（皮尔森相关系数为0.83（E1），0.84（E2）和0.94（E3））。多元回归分析产生了方程，用于根据排尿率和妊娠期来估算E1，E2，E3，E4，GEN和DDZ的血清浓度，这是朝着非侵入性生物监测评估怀孕期间“增加的”雌激素性迈出的重要一步。