In this study, preparation of magnetic surface molecular imprinted polymer (MMIP) is employed for sensitive solid phase extraction and measurement of amitriptyline (AMT) by spectrophotometric technique. After polymerization, cavities in the polymer particles corresponding to the AMT were created by leaching the polymer by an appropriate solution. The synthesized nanoparticles of magnetic surface molecular imprinted polymer mesoparticles (Fe₃O₄@SiO₂–MIP) were characterized by transfer electron microscopy (TEM), vibrating sample magnetometer (VSM), Fourier transform infrared spectroscopy (FTIR), X-Ray Diffraction (XRD) and Brunauer–Emmett–Teller (BET). Also, the pH of zero-point charge (pH_zpc) for Fe₃O₄@SiO₂–MIP was determined with the pH drift method. The influence of various parameters such as pH, amount of polymer and contact time were investigated by an optimization technique named Box–Behnken design (BBD) and the proposed quadratic model was fitted very well with the experimental data. The validity of optimum parameters and the equation of parameters were investigated by analysis of variance (ANOVA). Also Scatchard isotherm was explored for the equilibrium adsorption investigation and finally, the prepared polymer was successfully applied to the selective identification and the determination of AMT in plasma samples.

在这项研究中，磁性表面分子印迹聚合物（MMIP）的制备用于灵敏固相萃取和分光光度法测量阿米替林（AMT）。聚合后，通过用适当的溶液浸出聚合物，在聚合物颗粒中形成与AMT相对应的空腔。利用转移电子显微镜（TEM），振动样品磁强计（VSM），傅立叶变换红外光谱（FTIR），X射线衍射对磁性表面分子印迹聚合物介孔颗粒（Fe ₃ O ₄ @SiO ₂ -MIP）的合成纳米颗粒进行表征。 （XRD）和Brunauer-Emmett-Teller（BET）。另外，Fe ₃ O的零点电荷的pH（pH _zpc）用pH漂移法测定₄ @SiO ₂ -MIP。通过名为Box–Behnken设计（BBD）的优化技术研究了pH，聚合物量和接触时间等各种参数的影响，并且所提出的二次模型与实验数据非常吻合。通过方差分析（ANOVA）研究了最优参数和参数方程的有效性。还研究了Scatchard等温线以进行平衡吸附研究，最后将制得的聚合物成功地用于血浆样品中AMT的选择性鉴定和测定。