AbstractThe authors describe an electrochemical sensor for the determination of 5-hydroxytryptamine (5-HT; serotonin). It is based on the use of platinum nanoparticles (PtNPs) coated with molecularly imprinted silica (MIS). The MIS-coated PtNPs were prepared from 5-HT, PtNPs, phenyltrimethoxysilane and tetraethoxysilane that act as template, support, functional monomer and cross-linker, respectively. The MIS-coated PtNPs were characterized by Fourier transfer infrared spectroscopy and transmission electron microscopy. The MIS-coated PtNPs were drop-cast onto a glassy carbon electrode (GCE) to obtain an electrochemical sensor for 5-HT which exhibited fast response and high recognition ability for 5-HT. The imprinting factor of this electrode for 5-HT is 4.12, which is higher than that for its analogs. Under optimized conditions and at a typical working potential of 0.29 V (vs. Hg/Hg2Cl2), the electrode has a linear response in the 0.05–80 μM 5-HT concentration range and a 0.02 μM detection limit. The electrode was successfully applied to the determination of 5-HT in human serum samples. Graphical AbstractMolecularly imprinted silica-coated platinum nanoparticles were prepared using surface imprinting technique. A glassy carbon electrode modified with the surface imprinted material exhibits fast response in detecting 5-hydroxytryptamine, a low detection limit, good selectivity, reproducibility and stability.

中文翻译：

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基于使用分子印迹二氧化硅包覆的铂纳米颗粒的伏安法测定 5-羟色胺

摘要作者描述了一种用于测定 5-羟色胺（5-HT；5-羟色胺）的电化学传感器。它基于使用涂有分子印迹二氧化硅 (MIS) 的铂纳米粒子 (PtNP)。MIS 包覆的 PtNPs 由 5-HT、PtNPs、苯基三甲氧基硅烷和四乙氧基硅烷分别作为模板、载体、功能单体和交联剂制备。MIS 涂层 PtNPs 的特点是傅立叶转移红外光谱和透射电子显微镜。将涂有 MIS 的 PtNPs 滴铸到玻碳电极 (GCE) 上以获得对 5-HT 具有快速响应和高识别能力的 5-HT 电化学传感器。该电极对 5-HT 的压印因子为 4.12，高于其类似物。在优化条件和 0.29 V（相对于 Hg/Hg2Cl2）的典型工作电位下，电极在 0.05–80 μM 5-HT 浓度范围内具有线性响应，检测限为 0.02 μM。该电极成功应用于人血清样品中5-HT的测定。图形摘要采用表面印迹技术制备了分子印迹二氧化硅包覆的铂纳米粒子。用表面印迹材料修饰的玻碳电极检测5-羟色胺反应快，检出限低，选择性好，重现性和稳定性好。图形摘要采用表面印迹技术制备了分子印迹二氧化硅包覆的铂纳米粒子。用表面印迹材料修饰的玻碳电极检测5-羟色胺反应快，检出限低，选择性好，重现性和稳定性好。图形摘要采用表面印迹技术制备了分子印迹二氧化硅包覆的铂纳米粒子。用表面印迹材料修饰的玻碳电极检测5-羟色胺反应快，检出限低，选择性好，重现性和稳定性好。