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Cells on hierarchically-structured platforms hosting functionalized nanoparticles†
Biomaterials Science ( IF 5.8 ) Pub Date : 2018-03-13 00:00:00 , DOI: 10.1039/c7bm00904f
Chara Simitzi 1, 2, 3, 4 , Pascal Harimech 5, 6, 7, 8 , Syrago Spanou 1, 2, 3, 4, 9 , Christina Lanara 1, 2, 3, 4, 10 , Amelie Heuer-Jungemann 5, 6, 7, 8 , Aleka Manousaki 1, 2, 3, 4 , Costas Fotakis 1, 2, 3, 4, 11 , Anthi Ranella 1, 2, 3, 4 , Antonios G. Kanaras 5, 6, 7, 8, 12 , Emmanuel Stratakis 1, 2, 3, 4, 10
Affiliation  

In this work, we report on a novel approach to develop hierarchically-structured cell culture platforms incorporating functionalized gold nanoparticles (AuNPs). In particular, the hierarchical substrates comprise primary pseudo-periodic arrays of silicon microcones combined with a secondary nanoscale pattern of homogeneously deposited AuNPs terminated with bio-functional moieties. AuNPs with various functionalities (i.e. oligopeptides, small molecules and oligomers) were successfully attached onto the microstructures. Experiments with PC12 cells on hierarchical substrates incorporating AuNPs carrying the RGD peptide showed an impressive growth and NGF-induced differentiation of the PC12 cells, compared to that on the NP-free, bare, micropatterned substrates. The exploitation of the developed methodology for the binding of AuNPs as carriers of specific bio-functional moieties onto micropatterned culture substrates for cell biology studies is envisaged.

中文翻译:

托管功能化纳米粒子的分层结构平台上的细胞

在这项工作中,我们报告了一种新颖的方法,以开发结合功能化的金纳米颗粒(AuNPs)的分层结构的细胞培养平台。特别地,分层衬底包括硅微锥的主要伪周期阵列,其结合有以生物功能部分终止的均匀沉积的AuNP的次级纳米级图案。具有各种功能的AuNP(寡肽,小分子和寡聚体)成功连接到微结构上。与不含NP,裸露的微图案底物的PC12细胞在带有载有RGD肽的AuNP的分层底物上进行的实验相比,PC12细胞具有令人印象深刻的生长和NGF诱导的分化。设想开发用于将AuNP作为特定生物功能部分的载体结合到微图案化培养底物上的用于细胞生物学研究的方法学。
更新日期:2018-03-13
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