Triazole contaminants in water and soil environments can form complexes with metal ions, and therefore affect the bioavailability and toxicity of some heavy metals. In present study, significant increase of copper (Cu) uptake by earthworm (Eisenia fetida) was observed when combined pollution of benzotriazole (BTR) presented in soil. For instance, Cu accumulation in earthworms increased 55% approximately when BTR presented at the BTR/Cu molar ratio of 1:2.5. While the single Cu exposure (at 32 mg kg^-1 in soil) resulted in increased malondialdehyde (MDA) content in earthworms from 0.319 to 0.668 nmol·mg protein^-1, joint exposure to BTR at BTR/Cu molar ratio of 1:10 significantly decreased the MDA content to 0.405 nmol·mg protein^-1. This indicates a potential detoxification effect of BTR to Cu induced oxidative damage in earthworms. Varied Cu subcellular distribution can be observed in earthworms of the single and combined exposure treatments. With the combined exposure of BTR, the proportion of Cu associated with granular fraction, the toxically inert fraction in earthworms, increased from 25% to 39%. This phenomenon can be used to explain the protective effects of BTR against oxidative damage.

水和土壤环境中的三唑污染物会与金属离子形成络合物，因此会影响某些重金属的生物利用度和毒性。在本研究中，当土壤中存在苯并三唑（BTR）的综合污染时，worm（Eisenia fetida）对铜（Cu）的吸收显着增加。例如，当BTR / Cu摩尔比为1：2.5时，earth中的Cu积累大约增加了55％。虽然单次暴露于铜（在土壤中为32 mg kg ^-1）导致earth中的丙二醛（MDA）含量从0.319增加到0.668 nmol·mg蛋白^-1，但以BTR / Cu摩尔比为1:10联合暴露于BTR MDA含量显着降低至0.405 nmol·mg蛋白^-1。这表明BTR对earth诱导的Cu氧化损伤具有潜在的解毒作用。在earth的单一和联合暴露处理中，可以观察到铜亚细胞分布的变化。随着BTR的联合暴露，earth中与有毒惰性成分即颗粒状部分相关的Cu的比例从25％增加到39％。这种现象可以用来解释BTR对氧化损伤的保护作用。