Trypanosoma cruzi parasites are the causative agents of Chagas disease. These parasites infect cardiac and gastrointestinal tissues, leading to local inflammation and tissue damage. Digestive Chagas disease is associated with perturbations in food absorption, intestinal traffic and defecation. However, the impact of T. cruzi infection on the gut microbiota and metabolome have yet to be characterized. In this study, we applied mass spectrometry-based metabolomics and 16S rRNA sequencing to profile infection-associated alterations in fecal bacterial composition and fecal metabolome through the acute-stage and into the chronic stage of infection, in a murine model of Chagas disease. We observed joint microbial and chemical perturbations associated with T. cruzi infection. These included alterations in conjugated linoleic acid (CLA) derivatives and in specific members of families Ruminococcaceae and Lachnospiraceae, as well as alterations in secondary bile acids and members of order Clostridiales. These results highlight the importance of multi-‘omics’ and poly-microbial studies in understanding parasitic diseases in general, and Chagas disease in particular.

克氏锥虫寄生虫是南美锥虫病的病原体。这些寄生虫感染心脏和胃肠道组织，导致局部炎症和组织损伤。消化性恰加斯病与食物吸收，肠运输和排便的紊乱有关。但是，T的影响。肠道微生物群和代谢组的克鲁兹感染尚未得到鉴定。在这项研究中，我们应用了基于质谱的代谢组学和16S rRNA测序技术，在南美锥虫病的鼠模型中，分析了感染相关的粪便细菌组成和粪便代谢组在急性期和慢性期的变化。我们观察到与T相关的关节微生物和化学扰动。克鲁兹感染。在共轭亚油酸（CLA）的衍生物和在家庭中的特定成员这些包括改变Ruminococcaceae和毛螺，以及在次级胆汁酸和顺序梭菌的成员改变。这些结果凸显了多组学和多微生物研究在总体上了解寄生虫病，尤其是查加斯病的重要性。