Irradiation can significantly improve the incorporation of glucose into protein. In this study, the efficacy on conformation change and glycation extent of ovalbumin (OVA) powder was investigated during Co-60 irradiation treatment. Spectrophotometer was used to monitor the conformation and structure change of glycation OVA under various irradiation doses condition. A dose dependent increase in UV absorbance and development of fluorescence was observed. Free amino groups content showed that up to 5 kGy irradiation could accelerate the glycation reaction between OVA and glucose, while there were inapparent differences among the other various doses. Intrinsic, synchronous and 3D fluorescence spectra showed that the glycation reaction caused the dynamic quenching of OVA and changed the polarity of the hydrophobic microenvironment (Trp and Tyr residues) of OVA with unfolding conformation change.

辐照可以显着改善葡萄糖向蛋白质中的掺入。在这项研究中，研究了在Co-60辐照处理期间卵白蛋白（OVA）粉末对构象变化和糖基化程度的功效。用分光光度计监测在各种辐照剂量条件下糖基化OVA的构象和结构变化。观察到紫外线吸收的剂量依赖性增加和荧光的发展。游离氨基含量表明，高达5 kGy的辐射可以加速OVA和葡萄糖之间的糖基化反应，而其他各种剂量之间没有明显的差异。固有的，