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Squaramide-Based Supramolecular Materials for Three-Dimensional Cell Culture of Human Induced Pluripotent Stem Cells and Their Derivatives.
Biomacromolecules ( IF 5.5 ) Pub Date : 2018-03-12 , DOI: 10.1021/acs.biomac.7b01614 Ciqing Tong 1 , Tingxian Liu 1 , Victorio Saez Talens 1 , Willem E M Noteborn 1 , Thomas H Sharp 2 , Marco M R M Hendrix 3 , Ilja K Voets 3 , Christine L Mummery 4 , Valeria V Orlova 4 , Roxanne E Kieltyka 1
Biomacromolecules ( IF 5.5 ) Pub Date : 2018-03-12 , DOI: 10.1021/acs.biomac.7b01614 Ciqing Tong 1 , Tingxian Liu 1 , Victorio Saez Talens 1 , Willem E M Noteborn 1 , Thomas H Sharp 2 , Marco M R M Hendrix 3 , Ilja K Voets 3 , Christine L Mummery 4 , Valeria V Orlova 4 , Roxanne E Kieltyka 1
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Synthetic hydrogel materials can recapitulate the natural cell microenvironment; however, it is equally necessary that the gels maintain cell viability and phenotype while permitting reisolation without stress, especially for use in the stem cell field. Here, we describe a family of synthetically accessible, squaramide-based tripodal supramolecular monomers consisting of a flexible tris(2-aminoethyl)amine (TREN) core that self-assemble into supramolecular polymers and eventually into self-recovering hydrogels. Spectroscopic measurements revealed that monomer aggregation is mainly driven by a combination of hydrogen bonding and hydrophobicity. The self-recovering hydrogels were used to encapsulate NIH 3T3 fibroblasts as well as human-induced pluripotent stem cells (hiPSCs) and their derivatives in 3D. The materials reported here proved cytocompatible for these cell types with maintenance of hiPSCs in their undifferentiated state essential for their subsequent expansion or differentiation into a given cell type and potential for facile release by dilution due to their supramolecular nature.
中文翻译：
用于人类诱导多能干细胞及其衍生物三维细胞培养的方酰胺基超分子材料。
合成水凝胶材料可以重现自然细胞微环境；然而，同样有必要的是，凝胶保持细胞活力和表型，同时允许在没有压力的情况下重新分离，特别是用于干细胞领域。在这里，我们描述了一系列可合成的、基于方酰胺的三足超分子单体，由柔性三（2-氨基乙基）胺（TREN）核心组成，可自组装成超分子聚合物，并最终形成自我恢复的水凝胶。光谱测量表明单体聚集主要是由氢键和疏水性的组合驱动的。自我恢复水凝胶用于封装 NIH 3T3 成纤维细胞以及人诱导多能干细胞 (hiPSC) 及其 3D 衍生物。本文报道的材料证明与这些细胞类型具有细胞相容性，能够将 hiPSC 维持在未分化状态，这对于它们随后扩增或分化为给定细胞类型至关重要，并且由于其超分子性质，有可能通过稀释轻松释放。
更新日期：2018-03-12
中文翻译：
用于人类诱导多能干细胞及其衍生物三维细胞培养的方酰胺基超分子材料。
合成水凝胶材料可以重现自然细胞微环境；然而，同样有必要的是，凝胶保持细胞活力和表型，同时允许在没有压力的情况下重新分离，特别是用于干细胞领域。在这里，我们描述了一系列可合成的、基于方酰胺的三足超分子单体，由柔性三（2-氨基乙基）胺（TREN）核心组成，可自组装成超分子聚合物，并最终形成自我恢复的水凝胶。光谱测量表明单体聚集主要是由氢键和疏水性的组合驱动的。自我恢复水凝胶用于封装 NIH 3T3 成纤维细胞以及人诱导多能干细胞 (hiPSC) 及其 3D 衍生物。本文报道的材料证明与这些细胞类型具有细胞相容性，能够将 hiPSC 维持在未分化状态，这对于它们随后扩增或分化为给定细胞类型至关重要，并且由于其超分子性质，有可能通过稀释轻松释放。
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