In this paper we propose an easy way to detect the glycated form of human serum albumin which is biomarker for several diseases such as diabetes and Alzheimer. The detection platform is a label free impedimetric immunosensor, in which we used a monoclonal human serum albumin antibody as a bioreceptor and electrochemical impedance as a transducing method. The antibody was deposited onto a gold surface by simple physisorption technique. Bovine serum albumin was used as a blocking agent for non-specific binding interactions. Cyclic voltammetry and electrochemical impedance spectroscopy were used for the characterization of each layer. Human serum albumin was glycated at different levels with several concentrations of glucose ranging from 0 mM to 500 mM representing physiological, pathological (diabetic albumin) and suprapathological concentration of glucose. Through the calibration curves, we could clearly distinguish between two different areas related to physiological and pathological albumin glycation levels. The immunosensor displayed a linear range from 7.49% to 15.79% of glycated albumin to total albumin with a good sensitivity. Surface plasmon resonance imaging was also used to characterize the developed immunosensor.

在本文中，我们提出了一种检测人血清白蛋白糖化形式的简单方法，它是糖尿病和阿尔茨海默病等多种疾病的生物标志物。检测平台是无标记的阻抗免疫传感器，其中我们使用单克隆人血清白蛋白抗体作为生物受体和电化学阻抗作为转导方法。通过简单的物理吸附技术将抗体沉积在金表面上。牛血清白蛋白用作非特异性结合相互作用的阻断剂。循环伏安法和电化学阻抗谱用于表征每一层。人血清白蛋白在不同水平上被糖化，葡萄糖浓度从 0 mM 到 500 mM 不等，代表生理、葡萄糖的病理（糖尿病白蛋白）和超病理浓度。通过校准曲线，我们可以清楚地区分与生理和病理白蛋白糖化水平相关的两个不同区域。免疫传感器显示出从 7.49% 到 15.79% 的糖化白蛋白与总白蛋白的线性范围，具有良好的灵敏度。表面等离子共振成像也用于表征开发的免疫传感器。