In this study, we developed a simple and sensitive reversed phase ion-pair chromatographic method for the analysis of C4-C6 sugar alcohols. The method is based on the on-line complexation of sugar alcohols with molybdate ion. The resulting dinuclear anionic complexes can be separated on a reversed-phase C18 column using tetrabutylammonium chloride as an ion-pairing reagent. The mobile phase (pH 3.1) consisted of 0.1 mM disodium molybdate, 1 mM hydrochloric acid and 0.4 mM tetrabutylammonium chloride – 10% v/v methanol. By complexing with molybdate ion, sugar alcohols can be detected by their UV absorption at 247 nm with high resolution and sensitivity. The quantification limits of the examined sugar alcohols calculated at S/N = 10 were 0.1 mM for erythritol and xylitol and 0.01 mM for arabitol, sorbitol, mannitol and dulcitol. The detector response was linear over three orders of magnitude of sugar alcohol concentration. The proposed method was successfully applied to measure sugar alcohols in health drinks, eyedrops and mouthwashes.

在这项研究中，我们开发了一种简单而灵敏的反相离子对色谱法，用于分析C4-C6糖醇。该方法基于糖醇与钼酸根离子的在线络合。可以使用四丁基氯化铵作为离子对试剂，在反相C18色谱柱上分离所得的双核阴离子络合物。流动相（pH 3.1）由0.1 mM钼酸二钠，1 mM盐酸和0.4 mM氯化四丁铵– 10％v / v甲醇组成。通过与钼酸根离子络合，糖醇可以通过在247 nm处的紫外线吸收以高分辨率和高灵敏度检测出来。在S / N = 10时计算得出的糖醇的定量限为赤藓糖醇和木糖醇为0.1 mM，阿拉伯糖醇，山梨糖醇，甘露糖醇和二糖醇为0.01 mM。检测器的响应在糖醇浓度的三个数量级上呈线性。所提出的方法已成功地用于测量健康饮料，眼药水和漱口水中的糖醇。