The present research is focused on the use of a triple quadrupole mass spectrometer (QqQ MS) coupled with flow modulated comprehensive two-dimensional gas chromatography (FM GC × GC) for a multilevel elucidation of biodegradation potentiality of natural marine microbial populations during a bioremediation (biostimulation) treatment. The crude oil used for the evaluation of the bioremediation process, namely Dansk Blend Pier E1, represents a very complex sample. Hence, in order to understand the metabolic activity of microbial populations during the bioremediation process, a GC × GC system was used. The high separation power has allowed a detailed characterization of the different chemical families; moreover, thanks to the high acquisition frequency of the QqQ MS spectrometer, both full scan and multiple reaction monitoring (MRM) data were acquired in the same run. By using this system, both qualitative analysis of untargeted hydrocarbons mixture (crude oil) and qualitative analysis of biomarker compounds, present in low amount and often hindered under the bulk of the sample (i.e. adamantanes, diamantanes, steranes and hopanes), were performed simultaneously.

The bioremediation capability of biostimulated bacteria was evaluated at four (T₄), eight (T₈) and fourteen (T₁₄) days. Progressive degradation of linear, branched, and aromatic hydrocarbons, adamantanes, and diamantanes has been showed, whereas, results underline the lack of any kind of activity against steranes, and hopanes.

本研究的重点是使用三重四极杆质谱仪（QqQ MS）结合流量调制综合二维气相色谱仪（FM GC×GC）进行生物修复过程中多级分析天然海洋微生物种群的生物降解潜能（生物刺激）治疗。用于评估生物修复过程的原油，即Dansk Blend Pier E1，代表了非常复杂的样品。因此，为了了解生物修复过程中微生物种群的代谢活性，使用了GC×GC系统。高分离力可以对不同化学族进行详细表征。此外，由于QqQ MS光谱仪的高采集频率，在同一运行中同时获取了全扫描和多反应监测（MRM）数据。通过使用该系统，同时进行了少量的，经常受阻于样品主体（即金刚烷，二金刚烷，甾烷和hop烷）的非目标烃混合物（原油）的定性分析和生物标志物化合物的定性分析。 。

在四（T ₄），八（T ₈）和十四（T ₁₄）天评估了生物刺激细菌的生物修复能力。已经显示出线性，支链和芳族烃，金刚烷和二金刚烷的逐步降解，而结果表明缺乏对甾烷和hop烷的任何类型的活性。