Hard tissue implant materials which can cause a suitable alkaline microenvironment are thought to be beneficial for stimulating osteoblast differentiation while suppressing osteoclast generation. To make the local pH around the interface between materials and cells controllable, we prepared a series of Mg–Fe layered double hydroxide (LDH) films on acid-etched pure titanium surfaces via hydrothermal treatment. By adjusting the Mg/Fe proportion ratio, the interlayer spacing of Mg–Fe LDHs was regulated, making their OH⁻ exchange abilities adjustable, and this ultimately resulted in a microenvironment with a controllable pH value. In vitro experiments demonstrated that the Mg–Fe LDH film-modified titanium surface possessed good biocompatibility and osteogenic activity, especially the Mg–Fe LDH film with Mg/Fe proportion ratio of 4, which could form a suitable alkaline microenvironment for the growth and osteogenetic differentiation of stem cells. These results demonstrate the potential application of the prepared Mg–Fe LDH films in enhancing the osteogenesis of implant materials while providing a new way into the design of controllable alkaline environment.

中文翻译：

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通过Mg-Fe层状双氢氧化物膜 调节钛的局部pH值以增强成骨作用†

可以导致合适的碱性微环境的硬组织植入物材料被认为有利于刺激成骨细胞分化，同时抑制破骨细胞的产生。为了使材料与细胞之间的界面周围的局部pH值可控，我们通过水热处理在酸蚀纯钛表面上制备了一系列Mg-Fe层状双氢氧化物（LDH）膜。通过调整所述Mg / Fe的比例比，间距镁-铁水滑石的层间被调节，使得他们的OH ^-交换能力调节的，并且这最终导致具有可控pH值下具有的微环境。体外实验表明，Mg-Fe LDH膜修饰的钛表面具有良好的生物相容性和成骨活性，特别是Mg / Fe比例为4的Mg-Fe LDH膜可以形成合适的碱性微环境，促进了镁的生长和成骨分化。干细胞。这些结果证明了所制备的Mg-Fe LDH膜在增强植入物材料的成骨性方面的潜在应用，同时为可控碱性环境的设计提供了新途径。