Aluminum salt (Alum) is one of the most important immune adjuvants approved for use in humans, however it is not suitable for vaccination against various chronic infectious diseases and cancers for not being able to induce cell-mediated (Th1) immunity. Here, we encapsulated an Alum colloid inside β-glucan particles (GPs), which are a type of natural particles derived from the yeast glucan shells, to prepare hybrid GP-Alum (GP-Al) adjuvant particles with a very uniform size of 2–4 μm. These hybrid particles can be used to load antigen proteins through a simple mixing procedure, and can be highly specifically targeted to antigen-presenting cells (APCs) and strongly activate dendritic cells (DCs) maturation and cytokine secretion. In an animal model, they elicit a strong Th1-biased immune response and extremely high antibody titer, and cause marked prophylactic and therapeutic effects against tumors. As Alum has been proven to be a safe adjuvant to induce strong humoral responses and β-glucans are safe for human use, this very uniform hybrid Alum particulate system could have important application as a vaccine carrier to stimulate humoral and cellular immune responses at the same time.

铝盐（明矾）是已批准用于人类的最重要的免疫佐剂之一，但由于不能诱导细胞介导的（Th1）免疫，因此不适合针对各种慢性传染病和癌症进行疫苗接种。在这里，我们封装明矾胶体内β -葡聚糖颗粒（GPs）是一种来源于酵母葡聚糖壳的天然颗粒，可制备大小均匀的2-4μm杂种GP-明矾（GP-Al）佐剂颗粒。这些杂种颗粒可用于通过简单的混合过程加载抗原蛋白，并且可以高度特异性地靶向抗原呈递细胞（APC）并强烈激活树突状细胞（DC）成熟和细胞因子分泌。在动物模型中，它们引起强烈的Th1偏向免疫反应和极高的抗体滴度，并引起针对肿瘤的显着预防和治疗作用。由于明矾已被证明是诱导强烈体液反应的安全佐剂，而β-葡聚糖可安全用于人类，