Loss of function of a rice TPR-domain RNA-binding protein confers broad-spectrum disease resistance [Plant Biology],Proceedings of the National Academy of Sciences of the United States of America

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Loss of function of a rice TPR-domain RNA-binding protein confers broad-spectrum disease resistance [Plant Biology]
Proceedings of the National Academy of Sciences of the United States of America ( IF 9.4 ) Pub Date : 2018-03-20 00:00:00 , DOI: 10.1073/pnas.1705927115
Xiaogang Zhou _{1,

2,

3,

4} , Haicheng Liao _{1,

2,

3} , Mawsheng Chern _{5,

6} , Junjie Yin _{1,

2,

3} , Yufei Chen _{1,

2,

3} , Jianping Wang _{1,

2,

3} , Xiaobo Zhu _{1,

2,

3} , Zhixiong Chen _{1,

2,

3} , Can Yuan _{1,

2,

3} , Wen Zhao _{1,

2,

3} , Jing Wang _{1,

2,

3} , Weitao Li _{1,

2,

3} , Min He _{1,

2,

3} , Bingtian Ma _{1,

2,

3} , Jichun Wang _{1,

2,

3} , Peng Qin _{1,

2,

3} , Weilan Chen _{1,

2,

3} , Yuping Wang _{1,

2,

3} , Jiali Liu _{1,

2,

3} , Yangwen Qian ₇ , Wenming Wang _{1,

2,

3} , Xianjun Wu _{1,

2,

3} , Ping Li _{1,

2,

3} , Lihuang Zhu _{8,

9} , Shigui Li _{1,

2,

3} , Pamela C. Ronald _{5,

6} , Xuewei Chen _{1,

2,

3}

Affiliation

Crops carrying broad-spectrum resistance loci provide an effective strategy for controlling infectious disease because these loci typically confer resistance to diverse races of a pathogen or even multiple species of pathogens. Despite their importance, only a few crop broad-spectrum resistance loci have been reported. Here, we report the identification and characterization of the rice bsr-k1 (broad-spectrum resistance Kitaake-1) mutant, which confers broad-spectrum resistance against Magnaporthe oryzae and Xanthomonas oryzae pv oryzae with no major penalty on key agronomic traits. Map-based cloning reveals that Bsr-k1 encodes a tetratricopeptide repeats (TPRs)-containing protein, which binds to mRNAs of multiple OsPAL (OsPAL1–7) genes and promotes their turnover. Loss of function of the Bsr-k1 gene leads to accumulation of OsPAL1–7 mRNAs in the bsr-k1 mutant. Furthermore, overexpression of OsPAL1 in wild-type rice TP309 confers resistance to M. oryzae, supporting the role of OsPAL1. Our discovery of the bsr-k1 allele constitutes a significant conceptual advancement and provides a valuable tool for breeding broad-spectrum resistant rice.

中文翻译：

水稻TPR结构域RNA结合蛋白的功能丧失赋予广谱抗病性[植物生物学]

带有广谱抗性基因座的农作物提供了控制传染病的有效策略，因为这些基因座通常赋予对多种病原体甚至多种病原体的抗性。尽管具有重要意义，但仅报道了少数几个作物的广谱抗性基因座。在这里，我们报告水稻bsr-k1（广谱抗性Kitaake-1）突变体的鉴定和表征，该突变体赋予了对稻瘟病菌和米黄单胞菌PV稻的广谱抗性，而对关键农艺性状没有重大影响。基于图的克隆揭示了Bsr-k1编码含有四肽重复序列（TPRs）的蛋白质，该蛋白质与多个OsPAL（OsPAL1-7）基因的mRNA结合并促进其更新。Bsr-k1基因的功能丧失导致bsr-k1突变体中OsPAL1-7 mRNA的积累。此外，OsPAL1在野生型水稻TP309中的过表达赋予了对米曲霉的抗性，支持了OsPAL1的作用。我们对bsr-k1等位基因的发现构成了重要的概念进展，并为育种广谱抗性水稻提供了有价值的工具。

更新日期：2018-03-21

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