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Mitochondria-localising DNA-binding biscyclometalated phenyltriazole iridium(iii) dipyridophenazene complexes: syntheses and cellular imaging properties†
Dalton Transactions ( IF 3.5 ) Pub Date : 2018-03-12 00:00:00 , DOI: 10.1039/c8dt00046h Sreejesh Sreedharan 1 , Alessandro Sinopoli 2 , Paul J Jarman 3 , Darren Robinson 3 , Christopher Clemmet 4 , Paul A Scattergood 4 , Craig R Rice 4 , Carl G W Smythe 3 , James A Thomas 1 , Paul I P Elliott 4
Dalton Transactions ( IF 3.5 ) Pub Date : 2018-03-12 00:00:00 , DOI: 10.1039/c8dt00046h Sreejesh Sreedharan 1 , Alessandro Sinopoli 2 , Paul J Jarman 3 , Darren Robinson 3 , Christopher Clemmet 4 , Paul A Scattergood 4 , Craig R Rice 4 , Carl G W Smythe 3 , James A Thomas 1 , Paul I P Elliott 4
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Two new biscyclometalated complexes [Ir(ptzR)2(dppz)]+ (dppz = dipyridophenazene; ptzRH = 4-phenyl-1-benzyl-1,2,3-triazole (1+) and 4-phenyl-1-propyl-1,2,3-triazole (2+)) have been prepared. The hexafluorophosphate salts of these complexes have been fully characterized and, in one case, the X-ray structure of a nitrate salt was obtained. The DNA binding properties of the chloride salts of the complexes were investigated, as well as their cellular uptake by A2780 and MCF7 cell lines. Both complexes display an increase in the intensity of phosphorescence upon titration with duplex DNA, indicating the intercalation of the dppz ligand and, given that they are monocations, the complexes exhibit appreciable DNA binding affinity. Optical microscopy studies reveal that both complexes are taken up by live cancer cell lines displaying cytosol based luminescence. Colocalization studies with commercial probes show high Pearson coefficients with mitotracker dyes confirming that the new complexes specifically localize on mitochondria.
中文翻译：
线粒体定位 DNA 结合双环金属化苯基三唑铱 (iii) 双吡啶并吩嗪复合物：合成和细胞成像特性†
两个新的双环金属配合物 [Ir(ptz R ) 2 (dppz)] + (dppz = dipyridophenazene; ptz R H = 4-phenyl-1-benzyl-1,2,3-triazole ( 1 + ) 和 4-phenyl-1 -丙基-1,2,3-三唑 ( 2 +)) 已经准备好了。这些配合物的六氟磷酸盐已被充分表征，在一种情况下，获得了硝酸盐的 X 射线结构。研究了复合物的氯化物盐的 DNA 结合特性，以及它们被 A2780 和 MCF7 细胞系的细胞吸收。两种复合物在用双链 DNA 滴定时都显示出磷光强度的增加，表明 dppz 配体的嵌入，并且鉴于它们是单阳离子，复合物表现出明显的 DNA 结合亲和力。光学显微镜研究表明，这两种复合物都被显示出基于细胞质的发光的活癌细胞系所吸收。
更新日期：2018-03-12
中文翻译：
线粒体定位 DNA 结合双环金属化苯基三唑铱 (iii) 双吡啶并吩嗪复合物：合成和细胞成像特性†
两个新的双环金属配合物 [Ir(ptz R ) 2 (dppz)] + (dppz = dipyridophenazene; ptz R H = 4-phenyl-1-benzyl-1,2,3-triazole ( 1 + ) 和 4-phenyl-1 -丙基-1,2,3-三唑 ( 2 +)) 已经准备好了。这些配合物的六氟磷酸盐已被充分表征，在一种情况下，获得了硝酸盐的 X 射线结构。研究了复合物的氯化物盐的 DNA 结合特性，以及它们被 A2780 和 MCF7 细胞系的细胞吸收。两种复合物在用双链 DNA 滴定时都显示出磷光强度的增加，表明 dppz 配体的嵌入，并且鉴于它们是单阳离子，复合物表现出明显的 DNA 结合亲和力。光学显微镜研究表明，这两种复合物都被显示出基于细胞质的发光的活癌细胞系所吸收。
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