Novel and sensitive electrochemical sensor was fabricated for the assay of anti-HCV ledipasvir (LEDV) in different matrices. The designed sensor was based on 3D spinel ferromagnetic NiFe₂O₄ nanospheres and reduced graphene oxide (RGO) supported by morpholinium acid sulphate (MHS), as an ionic liquid (RGO/NSNiFe₂O₄/MHS). This sensor design was assigned to synergistically tailor the unique properties of nanostructured ferrites, RGO, and ionic liquid to maximize the sensor response. Electrode modification prevented aggregation of NiFe₂O_4, increasing electroactive surface area and allowed remarkable electro-catalytic oxidation of LEDV with an enhanced oxidation response. Differential pulse voltammetry was used for detection LEDV in complex matrices whereas; cyclic voltammetry and other techniques were employed to characterize the developed sensor properties. All experimental factors regarding sensor fabrication and chemical sensing properties were carefully studied and optimized. Under the optimum conditions, the designated sensor displayed a wide linear range (0.4–350 ng mL^-1) with LOD of 0.133 ng mL^-1. Additionally, the proposed sensor demonstrated good selectivity, stability and reproducibility, enabling the quantitative detection of LEDV in Harvoni^® tablets, human plasma and in a pharmacokinetic study. Our findings suggest that the developed sensor is a potential prototype material for fabrication of high-performance electrochemical sensors.

制备了新颖灵敏的电化学传感器，用于在不同基质中测定抗丙型肝炎病毒ledipasvir（LEDV）。设计的传感器基于3D尖晶石铁磁性NiFe ₂ O ₄纳米球和由吗啉酸硫酸盐（MHS）支撑的还原石墨烯（RGO），作为离子液体（RGO / NSNiFe ₂ O ₄ / MHS）。分配此传感器设计是为了协同调整纳米结构铁氧体，RGO和离子液体的独特性能，以使传感器响应最大化。电极改性可防止NiFe ₂ O ₄聚集增加了电活性表面积，并允许LEDV显着地电催化氧化，并具有增强的氧化反应。差分脉冲伏安法用于检测复杂基质中的LEDV。循环伏安法和其他技术被用来表征已开发的传感器特性。有关传感器制造和化学传感特性的所有实验因素均经过仔细研究和优化。在最佳条件下，指定的传感器显示出较宽的线性范围（0.4–350 ng mL ^-1），LOD为0.133 ng mL ^-1。此外，所提出的传感器表现出良好的选择性，稳定性和再现性，使LEDV在Harvoni定量检测^®片剂，人血浆和药代动力学研究。我们的发现表明，开发的传感器是用于制造高性能电化学传感器的潜在原型材料。