Flavonoid Glycosides from Fenugreek Seeds Regulate Glycolipid Metabolism by Improving Mitochondrial Function in 3T3-L1 Adipocytes in Vitro,Journal of Agricultural and Food Chemistry

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Flavonoid Glycosides from Fenugreek Seeds Regulate Glycolipid Metabolism by Improving Mitochondrial Function in 3T3-L1 Adipocytes in Vitro
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-03-10 00:00:00 , DOI: 10.1021/acs.jafc.8b00179
Guangxiang Luan _{1,

2,

3} , Yuwei Wang _{1,

2,

3} , Zhenhua Wang ₄ , Wenna Zhou _{1,

2,

3} , Na Hu _{1,

2} , Gang Li ₄ , Honglun Wang _{1,

2}

Affiliation

Fenugreek is a well known annual herb widely used in both medicine and food. Four flavonoid glycosides have been separated from fenugreek seeds in our previous study. In this study, the effects of the four flavonoid glycosides on regulating glycolipid metabolism and improving mitochondrial function were investigated. Isoorientin showed a very significant activity among these flavonoid glycosides. First, isoorientin decreased the accumulation of lipid droplets in 3T3-L1 preadipocytes by reducing the expression of adipokines including PPARγ, C/EBPα, and FAS. Second, isoorientin restored insulin-stimulated glucose uptake in dexamethasone-induced insulin-resistant 3T3-L1 adipocytes by reactivating Akt and AMPK. Finally, isoorientin improved mitochondrial dysfunction induced by dexamethasone in 3T3-L1 adipocytes. Isoorientin also reversed dexamethasone-induced decrease in mitochondrial membrane potential (MMP) and intracellular ATP production, reduced accumulation of intracellular reactive oxygen species (ROS), and protected mitochondrial DNA (mtDNA) from oxidative damage. At the same time, mitochondrial biogenesis is promoted. Therefore, isoorientin may be an attractive candidate as a glucose-lowering and insulin-resistance-improving agent for the treatment of diabetes.

中文翻译：

胡芦巴种子中的类黄酮糖苷通过改善体外3T3-L1脂肪细胞的线粒体功能来调节糖脂代谢。

胡芦巴是一种众所周知的一年生草本植物，广泛用于医学和食品中。在我们先前的研究中，已从胡芦巴种子中分离出四种黄酮苷。在这项研究中，研究了四种黄酮苷对调节糖脂代谢和改善线粒体功能的影响。异Orientinin在这些类黄酮糖苷中显示出非常重要的活性。首先，异Orientinin通过减少包括PPARγ，C /EBPα和FAS在内的脂肪因子的表达来减少3T3-L1前脂肪细胞中脂质小滴的积累。第二，异Orientinin通过重新激活Akt和AMPK，恢复了地塞米松诱导的胰岛素抵抗3T3-L1脂肪细胞中胰岛素刺激的葡萄糖摄取。最后，异Orientinin改善了地塞米松在3T3-L1脂肪细胞中诱导的线粒体功能障碍。异Orientin还逆转了地塞米松诱导的线粒体膜电位（MMP）和细胞内ATP产生的减少，细胞内活性氧（ROS）的积累减少以及保护了线粒体DNA（mtDNA）免受氧化损伤。同时，促进线粒体的生物发生。因此，异Orientinin可以作为治疗糖尿病的降糖和胰岛素抵抗改善药成为有吸引力的候选药物。

更新日期：2018-03-10

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