Cholesterol is widely present in animal fats and meat products and can undergo oxidation to form cholesterol-oxidation products (COPs) during heating. The objective of this study was to develop a QuEChERS method for the determination of COPs in edible animal fats and meat products via gas chromatography–mass spectrometry in which the required solvent volume and extraction time were reduced. By employing a DB-5MS capillary column (30 m × 0.25 mm i.d., 0.25 μm film thickness) and a temperature-programming method, seven COPs, cholesterol, and the internal standard 5α-cholestane could be separated within 19 min. The limits of detection and limits of quantitation based on the COP standards ranged from 0.16 to 180 ng/mL and from 0.32 to 400 ng/mL, respectively, and the recoveries ranged from 89.1 to 107.6% for boiled pork and from 80.5 to 105.6% for lard. The intraday variabilities for boiled pork and lard ranged from 2.27 to 6.87% and from 1.52 to 9.78%, respectively, whereas the interday variabilities ranged from 1.81 to 7.89% and from 3.57 to 9.26%, respectively. Among the various meat samples, fish showed the highest level of COPs (31.84 μg/g). For the edible fats, the COP contents in tallow (22.79–60.15 μg/g) were much higher than those in lard (0.152–2.55 μg/g) and butter (0.526–1.36 μg/g). Collectively, this method can be applied to determine COPs in cholesterol-containing foodstuffs.

中文翻译：

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QuEChERS结合气相色谱-质谱法测定肉类和动物脂肪中胆固醇氧化产物的改进分析方法

胆固醇广泛存在于动物脂肪和肉制品中，在加热过程中会发生氧化形成胆固醇氧化产物（COPs）。这项研究的目的是开发一种QuEChERS方法，通过气相色谱-质谱法测定食用动物脂肪和肉制品中的COP，减少了所需的溶剂量和提取时间。通过使用DB-5MS毛细管柱（内径30 m×0.25 mm，膜厚0.25μm）和温度编程方法，可以在19分钟内分离出7个COP，胆固醇和内标5α-胆甾烷。基于COP标准的检测限和定量限分别为0.16至180 ng / mL和0.32至400 ng / mL，煮熟猪肉的回收率为89.1至107.6％，而回收率为80.5至105。猪油的6％。猪肉和猪油的日间波动范围分别为2.27至6.87％和1.52至9.78％，而日间波动范围分别为1.81至7.89％和3.57至9.26％。在各种肉类样品中，鱼类的COP含量最高（31.84μg/ g）。对于食用脂肪，牛脂（22.79–60.15μg/ g）中的COP含量远高于猪油（0.152–2.55μg/ g）和黄油（0.526–1.36μg/ g）中的COP。总的来说，该方法可用于确定含胆固醇食品中的COP。对于食用脂肪，牛脂（22.79–60.15μg/ g）中的COP含量远高于猪油（0.152–2.55μg/ g）和黄油（0.526–1.36μg/ g）中的COP。总的来说，该方法可用于确定含胆固醇食品中的COP。对于食用脂肪，牛脂（22.79–60.15μg/ g）中的COP含量远高于猪油（0.152–2.55μg/ g）和黄油（0.526–1.36μg/ g）中的COP。总的来说，该方法可用于确定含胆固醇食品中的COP。