The objective of the present study was to explore the possibilities of the direct analysis of vegetable oils by normal-phase HPLC-DAD to evaluate the amounts of the main oxidation products of triacylglycerols containing linoleate, i.e. hydroperoxy-, keto- and hydroxy-dienes. A follow-up of oxidation at 40 °C of trilinolein, used as a simplified model lipid system, high-linoleic sunflower oil and high-oleic sunflower oil was performed to evaluate samples with different fatty acid compositions and different oxidation levels. The results showed that the HPLC-DAD method proposed allows for determining the concentrations of mono-hydroperoxydienes in edible oils without applying any isolation or derivatization step. The method was found to be direct, sensitive (LOQ 0.06 mmol/kg oil), precise (CV ≤ 5%) and also accurate, with 99% of analyte recovery. It also enabled the estimation of the minor amounts of ketodienes, but not those of hydroxydienes, which presented wide chromatographic bands and coeluted with a number of different minor oxidation compounds.

本研究的目的是探索通过正相HPLC-DAD直接分析植物油的可能性，以评估含亚油酸酯的三酰基甘油的主要氧化产物的量，即氢过氧-，酮-和羟基-二烯。在40°C下，对三亚油精（用作简化的模型脂质系统），高亚油酸葵花籽油和高油酸葵花籽油进行氧化跟踪，以评估具有不同脂肪酸组成和不同氧化水平的样品。结果表明，所提出的HPLC-DAD方法无需使用任何分离或衍生步骤即可测定食用油中单氢过氧二烯的浓度。发现该方法是直接的，灵敏的（LOQ 0.06 mmol / kg油），精确的（CV≤5％）并且也是准确的，分析物回收率为99％。