In view of the significance of glycoprotein biomarkers for early clinical diagnostics and treatments of diseases, it is essential to develop efficient and selective enrichment approaches for glycoproteins. Molecularly imprinted polymers (MIPs) have found important applications for separation and enrichment of glycoproteins. In this study, we use boronate affinity-based controllable oriented surface imprinting to prepare glycoprotein-imprinted magnetic nanoparticles. A glycoprotein was first immobilized onto the surface of boronic acid functionalized magnetic nanoparticles by boronate affinity. Subsequently, self-polymerization of 2-anilinoethanol was carried out to form thin imprinting coating on the magnetic nanoparticles surface with appropriate thickness. After removing the template with an acidic solution containing sodium dodecyl sulfate, 3D cavities complementary to the template were efficiently formed in the imprinting layer. The imprinting coating was highly hydrophilic and presented limited residual boronic acid, thus non-specific binding was avoided. Using horseradish peroxidase as a model target, the effects of imprinting conditions on the properties and performance of the prepared MIPs were investigated. The obtained MIPs exhibited several highly favorable features, including excellent specificity, high binding strength and low binding pH. The MIPs were successfully applied to the analysis of transferrin (TRF) in human serum.

鉴于糖蛋白生物标志物对疾病的早期临床诊断和治疗的重要性，必须开发有效且选择性的糖蛋白富集方法。分子印迹聚合物（MIP）已发现糖蛋白分离和富集的重要应用。在这项研究中，我们使用基于硼酸酯亲和力的可控定向表面印迹来制备糖蛋白印迹的磁性纳米颗粒。首先通过硼酸盐亲和力将糖蛋白固定在硼酸官能化的磁性纳米颗粒的表面上。随后，进行2-苯胺基乙醇的自聚合以在磁性纳米颗粒表面上以适当的厚度形成薄的压印涂层。用含有十二烷基硫酸钠的酸性溶液除去模板后，在压印层中有效地形成了与模板互补的3D腔。压印涂层是高度亲水的并且呈现出有限的残留硼酸，因此避免了非特异性结合。以辣根过氧化物酶为模型目标，研究了印迹条件对制备的MIPs性能和性能的影响。所得的MIP显示出几个高度有利的特征，包括优异的特异性，高结合强度和低结合pH。MIP已成功应用于人血清中转铁蛋白（TRF）的分析。以辣根过氧化物酶为模型目标，研究了印迹条件对制备的MIPs性能和性能的影响。所得的MIP显示出几个高度有利的特征，包括优异的特异性，高结合强度和低结合pH。MIP已成功应用于人血清中转铁蛋白（TRF）的分析。以辣根过氧化物酶为模型目标，研究了印迹条件对制备的MIPs性能和性能的影响。所得的MIP显示出几个高度有利的特征，包括优异的特异性，高结合强度和低结合pH。MIP已成功应用于人血清中转铁蛋白（TRF）的分析。