Miscanthus is a leading bioenergy crop and rice provides enormous biomass for biofuels. Using Calcofluor White staining, this work in situ observed an initial lignocellulose hydrolysis in two distinct Miscanthus accessions, rice cultivar (NPB), and Osfc16 mutant after mild chemical pretreatments. In comparison, the M. sin and Osfc16 respectively exhibited weak Calcofluor fluorescence compared to the M. sac and NPB during enzymatic hydrolysis, consistent with the high biomass saccharification detected in vitro. Using xyloglucan-directed monoclonal antibodies (mAbs), xyloglucan deconstruction was observed from initial cellulose hydrolysis, whereas the M. sin and Osfc16 exhibited relatively strong immunolabeling using xylan-directed mAb, confirming previous findings of xylan positive impacts on biomass saccharification. Furthermore, the M. sin showed quick disappearance of RG-I immunolabeling with varied HG labelings between acid and alkali pretreatments. Hence, this study demonstrated a quick approach to explore wall polymer distinct deconstruction for enhanced biomass saccharification under chemical pretreatment in bioenergy crops.

芒草是领先的生物能源作物，水稻为生物燃料提供了巨大的生物量。使用Calcofluor White染色，这项工作在温和的化学预处理后，在两个不同的芒草品种（水稻品种（NPB）和Osfc16突变体）中观察到了木质纤维素的初始水解。相比较而言，M.罪和Osfc16相比分别显示出弱卡尔科弗卢尔荧光M.囊酶水解期间和NPB，以高生物质糖化一致检测体外。使用木葡聚糖的单克隆抗体（mAb），由初始纤维素水解木葡聚糖观察解构，而M.罪和Osfc16使用木聚糖引导的mAb，证实对生物质糖化木聚糖积极影响以前的研究结果显示出相对强的免疫标记。此外，M.罪显示的RG-I与酸和碱预处理之间变化HG标号L免疫标记快速消失。因此，这项研究表明了一种快速的方法来探索壁聚合物独特的解构方法，以在生物能源作物中化学预处理下增强生物质的糖化作用。