Earthworms are common organisms in the soil toxicity-testing framework, and the epigeic Eisenia andrei and E. fetida are the recommended species. However, Eisenia species are rarely found in agricultural soils and recent studies have pointed out endogeic species are more sensitive to pesticide than Eisenia. Allolobophora chlorotica and Aporrectodea caliginosa are two endogeic soil-dwelling species that are abundant in the agroecosystem. However, knowledge on pesticide impact on this ecological group of earthworms is still incipient. Herein, we compared the biochemical (acetylcholinesterase [AChE] and carboxylesterase [CbE] activities) behavioral (burrowing, casting and feeding) biomarker responses of these two endogeic earthworm species exposed for 7 days to soils contaminated with 0.1, 1 and 10 mg kg⁻¹ ethyl-parathion. The results showed marked species-specific differences in both groups of biomarkers, suggesting A. caliginosa the most sensitive species to this organophosphorus pesticide under the exposure conditions in this study. Moreover, an in vitro inhibition trial with ethyl-paraoxon evidenced a higher sensitivity of A. caliginosa AChE activity compared with that of A. chlorotica. This finding suggested that this molecular target endpoint could contribute to the interspecific differences of behavioral responses rather than CbE activity; this latter considered a potent mechanism of OP removal. Our results suggest the inclusion of more than one endogeic earthworm species to assess toxicity from organophosphorus insecticides. However, the use of A. caliginosa in the environmental risk assessment framework of organophosphorus contamination is highly recommended because of its higher sensibility to this class of pesticides, in addition to its abundance in the agroecosystem.

在土壤毒性测试框架中，are是常见的生物，推荐的是大叶E（Eisenia andrei）和大肠埃希菌（E. fetida）。然而，蚯蚓种农业土壤和最近的研究很少发现人士指出endogeic种农药比更敏感赤子。绿叶异花青藻和caliporosa caliporosa是两个在农业生态系统中丰富的内生土壤居住物种。但是，关于农药对this这一生态组的影响的知识仍处于起步阶段。在这里，我们比较了这两种内生earth物种在受0.1、1和10 mg kg污染的土壤中暴露7天后的生化（乙酰胆碱酯酶[AChE]和羧酸酯酶[CbE]活性）行为（穴居，铸造和觅食）生物标志物响应^{- 1}乙基对硫磷。结果显示两组生物标记物之间存在明显的物种特异性差异，这表明在本研究中的暴露条件下，caliginosa A. caliginosa是对该有机磷农药最敏感的物种。而且，在体外用乙基对氧磷进行的抑制试验证明，与叶绿假单胞菌相比，caliginosa AChE活性具有更高的敏感性。这一发现表明，该分子靶标终点可能导致行为反应的种间差异，而不是CbE活性。后者被认为是去除OP的有效机制。我们的结果表明，包括了不止一种内生earth物种，以评估有机磷杀虫剂的毒性。但是，强烈建议在有机磷污染的环境风险评估框架中使用caliginosa菌，因为它除了在农业生态系统中含量较高外，对此类农药的敏感性更高。