Abstract The synthesis of a hyperbranched polyesteramide functionalized with 6 acridine groups (P1000-ACRID) is described, and its photophysical properties compared with its low molecular weight reference compound (ACRIDyne). The hyperbranched polymer does not show aggregation of the acridine groups neither in solution nor in solid polymer matrices. The behavior of both chromophores as fluorescent acid sensors has been studied in solution and in solid polymer films, showing an increase in their fluorescence emission. The stoichiometry of the protonation reaction has been calculated, showing that ACRYDyne is able to protonate twice both in solution and in solid polymer matrices, whereas P1000-ACRID only protonates once. The sensing sensitivity of the compounds is consistent with this. The antimicrobial activity of the hyperbranched polymer has been tested against five bacteria (Gram positive and Gram negative) and two yeasts, showing high antimicrobial activity. The biocompatibility of low- and high-molecular weight compounds has been studied towards two mammalian cell lines (C2C12 and Swiss3T3), and the hyperbranched polymer shows higher biocompatibility than its low-molecular weight reference compound. P1000-ACRID allows enough cellular viability when used in its biofunctional concentration range.

中文翻译：

---

新型荧光吖啶超支化聚合物的合成和光谱特性：在酸传感和抗菌剂中的应用

摘要 描述了具有 6 个吖啶基团功能化的超支化聚酯酰胺 (P1000-ACRID) 的合成，并将其光物理性质与其低分子量参考化合物 (ACRIDyne) 进行了比较。超支化聚合物在溶液和固体聚合物基质中均未显示吖啶基团的聚集。两种发色团作为荧光酸传感器的行为已经在溶液和固体聚合物薄膜中进行了研究，表明它们的荧光发射增加。已经计算了质子化反应的化学计量，表明 ACRYDyne 能够在溶液和固体聚合物基质中质子化两次，而 P1000-ACRID 只能质子化一次。化合物的传感灵敏度与此一致。超支化聚合物的抗菌活性已针对五种细菌（革兰氏阳性和革兰氏阴性）和两种酵母进行了测试，显示出高抗菌活性。已经针对两种哺乳动物细胞系（C2C12 和 Swiss3T3）研究了低分子量和高分子量化合物的生物相容性，超支化聚合物显示出比其低分子量参考化合物更高的生物相容性。P1000-ACRID 在其生物功能浓度范围内使用时可提供足够的细胞活力。并且超支化聚合物显示出比其低分子量参考化合物更高的生物相容性。P1000-ACRID 在其生物功能浓度范围内使用时可提供足够的细胞活力。并且超支化聚合物显示出比其低分子量参考化合物更高的生物相容性。P1000-ACRID 在其生物功能浓度范围内使用时可提供足够的细胞活力。