Abstract Here, we report a new method to conjugate nanoparticles with live cells with high efficiency and biocompatibility. Distearoylphosphatidylethanolamine (DSPE)-PEG polymers was first gifted with cyclodextrin and then anchored on the surface of mesenchymal stem cells (MSCs). Then azobenzene derivative (Azo)-modified upconversion nanoparticles were conjugated to cells to construct live cells-based nanoparticle delivery system by host-guest interactions between cyclodextrin and Azo. Owing to free of chemical reactions of the DSPE-anchoring and host-guest interactions process, the conjugating strategy is very biocompatible. In vitro study illustrated that the differentiation potential of stem cells was not affected after anchoring nanoparticles indicating a biocompatible conjugating method for the construction of cell patch.

中文翻译：

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使用上转换纳米颗粒-偶联间充质干细胞构建细胞贴片的生物相容性策略

摘要 在这里，我们报告了一种以高效和生物相容性将纳米颗粒与活细胞结合的新方法。二硬脂酰磷脂酰乙醇胺 (DSPE)-PEG 聚合物首先被赋予环糊精，然后锚定在间充质干细胞 (MSC) 的表面。然后，通过环糊精和偶氮之间的主客体相互作用，将偶氮苯衍生物（Azo）修饰的上转换纳米粒子与细胞结合，构建基于活细胞的纳米粒子传递系统。由于没有 DSPE 锚定和主客体相互作用过程的化学反应，共轭策略是非常生物相容的。体外研究表明，锚定纳米颗粒后，干细胞的分化潜能不受影响，表明这是一种构建细胞贴片的生物相容性偶联方法。