Abstract This study aimed to evaluate the safety of GM rice containing Cry1Ab protein (1.64 mg/kg) as the main ingredient in the diet after 360 days of feeding in the highly inbred Wuzhishan pigs (WZSP). A total of 28 pigs aged 3 months were divided into two groups and fed with either non-GM (MH86 group, female n = 7, male n = 7) or GM rice (Bt group, female n = 7, male n = 7). The total dosage of Cry1Ab protein for female and male pigs was 0.972 and 0.963 mg/kg body weight by the end of final weighing date (D 75), respectively. The results showed that there were no differences in the average daily gain, average daily food intake, body weight, feed conversion ratio, oestrus cycle duration, oestrus period, litter size, serum steroids, or the birth and weaning weight of offspring between Bt and MH86 groups. All pigs (n = 28) were sacrificed to collect tissues and blood samples for the analysis of relative organ weights, blood physiological and biochemical parameters and histopathological examinations. No significant differences existed in the tested indicators except that total protein was significantly higher in the MH86 compared to the Bt group (P 0.05). No pathologic abnormal changes were found from histopathological examinations of the main tissues. On the basis of these results, feeding with Bt rice for 360 days did not affect the growth, reproductive performance, hematology, or organ morphology in WZSP.

中文翻译：

---

长期饲喂转基因Bt水稻对高度自交五指山猪生长繁殖性能的影响

摘要 本研究旨在评价以Cry1Ab 蛋白(1.64 mg/kg) 为主要成分的转基因水稻在高度近交五指山猪(WZSP) 饲养360 天后的安全性。将 28 头 3 个月大的猪分为两组，分别饲喂非转基因（MH86 组，雌性 n = 7，雄性 n = 7）或转基因水稻（Bt 组，雌性 n = 7，雄性 n = 7） ）。到最后称重日期 (D 75) 结束时，雌性和雄性猪的 Cry1Ab 蛋白总剂量分别为 0.972 和 0.963 毫克/千克体重。结果表明，Bt和Bt小鼠在平均日增重、平均日摄食量、体重、饲料转化率、发情周期持续时间、发情期、窝产仔数、血清类固醇或出生和断奶体重等方面均无差异。 MH86 团体。处死所有猪（n = 28）以收集组织和血液样本，用于分析相对器官重量、血液生理生化参数和组织病理学检查。除了MH86的总蛋白显着高于Bt组（P <0.05）外，测试指标不存在显着差异。主要组织组织病理学检查未见病理异常变化。根据这些结果，饲喂 Bt 水稻 360 天不会影响 WZSP 的生长、繁殖性能、血液学或器官形态。除了MH86的总蛋白显着高于Bt组（P <0.05）外，测试指标不存在显着差异。主要组织组织病理学检查未见病理异常变化。根据这些结果，饲喂 Bt 水稻 360 天不会影响 WZSP 的生长、繁殖性能、血液学或器官形态。除了MH86的总蛋白显着高于Bt组（P <0.05）外，测试指标不存在显着差异。主要组织组织病理学检查未见病理异常变化。根据这些结果，饲喂 Bt 水稻 360 天不会影响 WZSP 的生长、繁殖性能、血液学或器官形态。