Transgenic approaches are instrumental for labeling and manipulating cells and cellular machineries in vivo. Transgenes have traditionally been static entities that remained unaltered following genome integration, limiting their versatility. The development of DNA recombinase-based methods to modify, excise, or rearrange transgene cassettes has introduced versatile control of transgene activity and function. In particular, recombinase-controlled transgenes enable regulation of exogenous gene expression, conditional mutagenesis, and genetic lineage tracing. In zebrafish, transgenesis-based recombinase genetics using Cre/lox, Flp/FRT, and ΦC31 are increasingly applied to study development and homeostasis, and to generate disease models. Intersected with the versatile imaging capacity of the zebrafish model and recent breakthroughs in genome editing, we review and discuss past, current, and potential future approaches and resources for recombinase-based techniques in zebrafish.

转基因方法在体内标记和操纵细胞和细胞机械方面发挥了重要作用。传统上，转基因是静态实体，在基因组整合后仍保持不变，从而限制了其多功能性。基于DNA重组酶的方法的改进，修饰或重排转基因盒的开发引入了对转基因活性和功能的通用控制。特别地，重组酶控制的转基因能够调节外源基因表达，条件诱变和遗传谱系追踪。在斑马鱼中，使用Cre / lox，Flp / FRT进行基于转基因的重组酶遗传和ΦC31越来越多地用于研究发育和体内平衡，并生成疾病模型。与斑马鱼模型的通用成像能力以及基因组编辑中的最新突破相交，我们回顾并讨论了斑马鱼中基于重组酶的技术的过去，当前和潜在的未来方法和资源。