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Cell-size confinement effect on protein diffusion in crowded poly(ethylene)glycol solution†
Physical Chemistry Chemical Physics ( IF 2.9 ) Pub Date : 2018-03-09 00:00:00 , DOI: 10.1039/c7cp08199e Chiho Watanabe 1, 2, 3, 4, 5 , Miho Yanagisawa 1, 2, 3, 4, 5
Physical Chemistry Chemical Physics ( IF 2.9 ) Pub Date : 2018-03-09 00:00:00 , DOI: 10.1039/c7cp08199e Chiho Watanabe 1, 2, 3, 4, 5 , Miho Yanagisawa 1, 2, 3, 4, 5
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Micrometric membrane confinements and macromolecular crowding of cytoplasm are key factors that regulate molecular diffusion in live cells. Previous studies have shown that macromolecular crowding delays molecular diffusion. However, the effect of cell-size confinement on diffusion in the crowding environment is yet to be elucidated. Using fluorescence correlation spectroscopy (FCS), we analyzed protein diffusion in microdroplets containing polymer solution covered with lipid membranes that mimic cells. As a result, we found that a synergistic condition of crowding and micrometric confinement results in accelerated protein diffusion on a sub-millisecond time scale. This acceleration rate strongly depended on the size of the confined space and the degree of crowding. These findings indicate that cell-size confinement supports protein diffusion in highly crowded cytoplasm.
中文翻译：
细胞大小限制对蛋白质在拥挤的聚（乙二醇）溶液中扩散的影响†
微米级的膜限制和细胞质的大分子拥挤是调节活细胞中分子扩散的关键因素。先前的研究表明，大分子拥挤延迟了分子扩散。然而，细胞大小限制对在拥挤环境中扩散的影响尚待阐明。使用荧光相关光谱法（FCS），我们分析了微滴中的蛋白质扩散，该微滴包含覆盖有模仿细胞的脂质膜的聚合物溶液。结果，我们发现拥挤和微米级限制的协同条件导致亚毫秒级的蛋白质加速扩散。该加速度很大程度上取决于密闭空间的大小和拥挤程度。
更新日期：2018-03-09
中文翻译：
细胞大小限制对蛋白质在拥挤的聚（乙二醇）溶液中扩散的影响†
微米级的膜限制和细胞质的大分子拥挤是调节活细胞中分子扩散的关键因素。先前的研究表明，大分子拥挤延迟了分子扩散。然而，细胞大小限制对在拥挤环境中扩散的影响尚待阐明。使用荧光相关光谱法（FCS），我们分析了微滴中的蛋白质扩散，该微滴包含覆盖有模仿细胞的脂质膜的聚合物溶液。结果，我们发现拥挤和微米级限制的协同条件导致亚毫秒级的蛋白质加速扩散。该加速度很大程度上取决于密闭空间的大小和拥挤程度。
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