Here, we demonstrate an easy-to-implement and general biosensing strategy by coupling the small-molecule recognition of the bacterial allosteric transcription factor (aTF) with isothermal strand displacement amplification (SDA) in vitro. Based on this strategy, we developed two biosensors for the detection of an antiseptic, p-hydroxybenzoic acid, and a disease marker, uric acid, using bacterial aTF HosA and HucR, respectively, highlighting the great potential of this strategy for the development of small-molecule biosensors.

中文翻译：

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通过将细菌变构转录因子的识别与等温链置换扩增相结合来开发小分子生物传感器†

在这里，我们通过将细菌变构转录因子（aTF）的小分子识别与体外等温链置换扩增（SDA）结合起来，展示了一种易于实现的通用生物传感策略。在此策略的基础上，我们开发了两种生物传感器，分别使用细菌aTF HosA和HucR来检测防腐剂对羟基苯甲酸和疾病标记物尿酸，突显了该策略在开发小型细菌方面的巨大潜力分子生物传感器。