The induction of RIBE (Radiation Induced Bystander Effect) is a non-target effect of low radiation doses that has already been verified at an inter-organismic level in fish and small mammals. Although the theoretical impact in the field of environmental risk assessment (ERA) is possible, there is a gap of knowledge regarding this phenomenon in invertebrate groups and following environmentally relevant exposures. To understand if RIBE should be considered for ERA of radionuclide-rich wastewaters, we exposed Daphnia magna (<24 h and 5d old) to a 2% diluted uranium mine effluent for 48 h, and to a matching dose of waterborne uranium (55.3 μg L⁻¹). Then the exposed organisms were placed (24 and 48 h) in a clean medium together with non-exposed neonates. The DNA damage observed for the non-exposed organisms was statistically significant after the 24 h cohabitation for both uranium (neonates p = 0.002; 5 d-old daphnids p = <0.001) and uranium mine effluent exposure (only for neonates p = 0.042). After 48 h cohabitation significant results were obtained only for uranium exposure (neonates p = 0.017; 5 d-old daphnids p = 0.013). Although there may be some variability associated to age and exposure duration, the significant DNA damage detected in non-exposed organisms clearly reveals the occurrence of RIBE in D. magna. The data obtained and here presented are a valuable contribution for the discussion about the relevance of RIBE for environmental risk assessment.

RIBE（辐射诱导的旁观者效应）的诱导是低辐射剂量的非目标效应，该效应已在鱼类和小型哺乳动物的生物间水平上得到证实。尽管在环境风险评估（ERA）领域有理论上的影响是可能的，但是在无脊椎动物群体中以及在与环境相关的暴露之后，关于这一现象的知识尚有不足。为了了解是否应将RIBE用于富含放射性核素废水的ERA，我们将Daphnia magna（<24小时和5d旧）暴露于2％稀释的铀矿废水中48小时，并暴露于相称剂量的水性铀（55.3μg L ^-1）。然后将暴露的生物与未暴露的新生儿一起放置在干净的培养基中（24和48小时）。铀（新生儿（p  = 0.002），5 d大水蚤（p  = <0.001）和铀矿山废水暴露（仅对于新生儿p = 0.042）同居24小时后，未暴露生物体观察到的DNA损伤在统计学上具有统计学意义。。同居48小时后，仅暴露于铀才获得了显着结果（新生儿酸盐p  = 0.017； 5 d大水蚤p = 0.013）。尽管可能与年龄和暴露时间有关，但是在未暴露生物中检测到的显着DNA损伤清楚地揭示了大果蝇中RIBE的发生。获得的数据和在此呈现的数据对于讨论RIBE与环境风险评估的相关性具有宝贵的贡献。