Hypoxia results when the oxygen supply to rapidly growing tumors becomes inadequate to support various physiological processes. This plays a role in tumor metastasis and treatment resistance. Therefore, identifying tumor hypoxia can guide treatment planning and predict patient responses. However, hypoxic volumes are heterogeneously dispersed throughout a tumor, making it a challenge to pinpoint them with any degree of accuracy. Herein, we report the development of ratiometric hypoxia probe 1 (rHyP-1), which is a hypoxia-responsive small-molecule probe designed for reliable hypoxia detection using photoacoustic imaging. Photoacoustic imaging utilizes near-infrared (NIR) light to induce the production of ultrasound signals, enabling high-resolution image acquisition at centimeter depths. Together with the ratiometric capability of rHyP-1, reliable hypoxia detection with unprecedented spatial resolution is possible while minimizing error associated with concentration dependence and tissue heterogeneity.

中文翻译：

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基于N氧化物的探针的光物理调谐使肿瘤缺氧的比例光声成像成为可能。

当对迅速生长的肿瘤的氧气供应不足以支持各种生理过程时，就会导致缺氧。这在肿瘤转移和治疗抵抗中起作用。因此，确定肿瘤缺氧可以指导治疗计划并预测患者反应。然而，低氧体积在整个肿瘤中是异质分布的，因此以任何精确度精确定位它们都是一个挑战。在这里，我们报告的发展[R atiometric HY poxia p长袍1（rHyP-1），这是一种对缺氧敏感的小分子探针，旨在通过光声成像可靠地检测缺氧。光声成像利用近红外（NIR）光来诱导超声信号的产生，从而能够在厘米深度处获取高分辨率图像。结合rHyP-1的比例功能，可以实现具有前所未有的空间分辨率的可靠缺氧检测，同时最大限度地减少与浓度依赖性和组织异质性相关的误差。