The fruit fly is a frequently used model system with a high degree of human disease-related genetic homology. The quantitative chemical analysis of fruit fly tissues and hemolymph uniquely brings chemical signaling and compositional information to fly experimentation. The work here explores the impact of measured chemical content of hemolymph with three aspects of sample collection and preparation. Cellular content of hemolymph was quantitated and removed to determine hemolymph composition changes for seven primary amine analytes. Hemolymph sampling methods were adapted to determine differences in primary amine composition of hemolymph collected from the head, antenna, and abdomen. Also, three types of anesthesia were employed with hemolymph collection to quantitate effects on measured amino acid content. Cell content was found to be 45.4 ± 22.1 cells/nL of hemolymph collected from both adult and larvae flies. Cell-concentrated fractions of adult, but not larvae, hemolymph were found to have higher and more variable amine content. There were amino acid content differences found between all three areas indicating a robust method to characterize chemical markers from specific regions of a fly, and these appear related to physiological activity. Methods of anesthesia have an impact on hemolymph amino acid composition related to overall physiological impact to fly including higher amino acid content variability and oxygen deprivation effects. Together, these analyses identify potential complications with Drosophila hemolymph analysis and opportunities for future studies to relate hemolymph content with model physiological activity.

中文翻译：

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采样和细胞分离对果蝇血淋巴中氨基酸测定的影响

果蝇是具有高度人类疾病相关遗传同源性的常用模型系统。对果蝇组织和血淋巴的定量化学分析将独特的化学信号和组成信息带入果蝇实验。本文的工作从样品收集和制备的三个方面探讨了血淋巴中化学成分含量的影响。定量和去除血淋巴的细胞含量以确定七种伯胺分析物的血淋巴组成变化。调整了血淋巴采样方法，以确定从头部，触角和腹部采集的血淋巴的伯胺成分差异。同样，采用三种麻醉方法进行血淋巴收集，以量化对测得的氨基酸含量的影响。发现细胞含量为45.4±22。从成年幼虫和幼虫中收集到1个细胞/ nL的血淋巴。发现成人的细胞浓缩级分（而不是幼虫的血淋巴）具有更高和更多的可变胺含量。在所有三个区域之间都发现了氨基酸含量差异，这表明一种可靠的方法可以表征果蝇特定区域的化学标记，并且这些标记似乎与生理活性有关。麻醉方法会对与飞行的整体生理影响有关的血淋巴氨基酸组成产生影响，包括更高的氨基酸含量变异性和氧气剥夺作用。这些分析共同确定了潜在的并发症 在所有三个区域之间都发现了氨基酸含量差异，这表明一种可靠的方法可以表征果蝇特定区域的化学标记，并且这些标记似乎与生理活性有关。麻醉方法会对与飞行的整体生理影响有关的血淋巴氨基酸组成产生影响，包括更高的氨基酸含量变异性和氧气剥夺作用。这些分析共同确定了潜在的并发症 在所有三个区域之间都发现了氨基酸含量差异，这表明一种可靠的方法可以表征果蝇特定区域的化学标记，并且这些标记似乎与生理活性有关。麻醉方法会对与飞行的整体生理影响有关的血淋巴氨基酸组成产生影响，包括更高的氨基酸含量变异性和氧气剥夺作用。这些分析共同确定了潜在的并发症 麻醉方法会对与飞行的整体生理影响有关的血淋巴氨基酸组成产生影响，包括更高的氨基酸含量变异性和氧气剥夺作用。这些分析共同确定了潜在的并发症 麻醉方法会对与飞行的整体生理影响有关的血淋巴氨基酸组成产生影响，包括更高的氨基酸含量变异性和氧气剥夺作用。这些分析共同确定了潜在的并发症果蝇血淋巴分析和将来的研究机会，以将血淋巴含量与模型的生理活性联系起来。