Lophiostoma bipolare was taxonomically revised based on the morphological observations and phylogenetic analyses of molecular data from nuclear rDNA SSU-ITS-LSU, TUB, tef1, and rpb2 genes. Twenty-nine strains were morphologically similar to Lo. bipolare. A total of 174 sequences were generated from the Lo. bipolare complex. Phylogenetic analyses based on TUB sequence revealed 11 distinct species within the Lo. bipolare complex. Morphological features of the ascospores and the anatomical structure of the ascomata from both field collections as well as axenic culture, which have been reported previously as variable features at intraspecific levels, were compared to evaluate the taxonomic reliability of these features. To clarify the generic position of the 11 species, phylogenetic analyses were done on SSU-ITS-LSU-tef1-rpb2 gene sequences. The Lo. bipolare complex shared phylogenetic relationships with Pseudolophiostoma and Vaginatispora, and formed an additional five distinct clades from other members of Lophiostomataceae. According to its phylogenetic position, Lo. bipolare sensu stricto was distantly related to Lophiostoma s. str., and formed an independent clade within Lophiostomataceae. Lophiostoma bipolare s. str. could be distinguished from the other lophiostomataceous genera by the clypeus around the ostiolar neck and by the thin and uniformly thick peridium. A novel genus described as Lentistoma was established to accommodate this species, and the epitypification of Lentistoma bipolare (basionym: Massarina bipolaris) was proposed. Other lineages of the Lo. bipolare complex could not be separated on the basis of the ascospore size and sheath variations, but were distinguished based on ascomatal features, such as the existence of the clypeus, brown hyphae surrounding the peridium, and the contexture of the peridium, which were stable indicators of generic boundaries in Lophiostomataceae. Four additional new genera with five new species were recognised based on these morphological differences: Crassiclypeus (C. aquaticus), Flabellascoma (F. cycadicola and F. minimum), Leptoparies (Lep. palmarum), and Pseudopaucispora (Pseudop. brunneospora). Three new species were added to Pseudolophiostoma (Pseudol. cornisporum, Pseudol. obtusisporum, and Pseudol. tropicum) and two new species were added to Vaginatispora (V. amygdali and V. scabrispora). The re-evaluation of the validity of several previously recognised genera resulted in the introduction of two new genera with new combinations for Lophiostoma pseudoarmatisporum as Parapaucispora pseudoarmatispora and Vaginatispora fuckelii as Neovaginatispora fuckelii.

根据核 rDNA SSU-ITS-LSU、 TUB 、 tef1和rpb2基因分子数据的形态学观察和系统发育分析，对Lophiostoma bipolare进行了分类学修订。 29 个菌株在形态上与Lo相似。双极。从Lo中总共生成了 174 个序列。双极复合体。基于TUB序列的系统发育分析揭示了Lo 内有 11 个不同的物种。双极复合体。对来自现场收集和无菌培养的子囊孢子的形态特征和子囊的解剖结构进行比较，以评估这些特征的分类学可靠性，这些特征先前已报道为种内水平的可变特征。为了阐明这 11 个物种的属位，对 SSU-ITS-LSU- tef1 - rpb2基因序列进行系统发育分析。洛.双极复合体与Pseudolophiostoma和Vaginatispora具有共同的系统发育关系，并与Lophiostomataceae的其他成员形成了另外 5 个不同的分支。根据其系统发育位置， Lo。双极严格意义上与Lophiostoma s 有远缘关系。 str.，并在Lophiostomataceae 内形成一个独立的分支。 Lophiostoma 双极s.海峡可以通过小孔颈周围的唇瓣和薄而均匀厚的周包膜与其他唇口科属区分开来。 一个被描述为Lentistoma 的新属被建立来容纳该物种，并提出了Lentistoma bipolare （基本名： Massarina bipolaris ）的表型化。洛氏的其他血统。双极复合体不能根据子囊孢子大小和鞘的变化来区分，但可以根据子囊特征来区分，例如唇部的存在、包膜周围的棕色菌丝以及包膜的结构，这些都是稳定的指标Lophiostomataceae属的界线。基于这些形态差异，另外四个新属和五个新种被识别： Crassiclypeus ( C. Aquaticus )、 Flabellascoma ( F. cycadicola和F.minimum )、 Leptoparies ( Lep . palmarum ) 和Pseudopaucispora ( Pseudop . brunneospora )。 Pseudolophiostoma中添加了 3 个新种（ Pseudol . cornisporum 、 Pseudol . obtusisporum和Pseudol.tropicum ） ， Vaginatispora中添加了 2 个新种（ V. amygdali和V. scabrispora ）。对先前认可的几个属的有效性的重新评估导致引入了两个新属，其中Lophiostoma pseudoarmatisporum为Parapaucispora pseudoarmatispora ， Vaginatispora funkelii为Neovaginatispora funkelii 。